In vitro IN VITRO EVALUATION OF THE ANTIBACTERIAL EFFICACY OF DATURA STRAMONIUM LEAF AGAINST HELICOBACTER PYLORI
DOI:
https://doi.org/10.33003/fjs-2024-0806-2845Keywords:
Antimicrobial resistance, Antibacterial activity, Datura stramonium, Helicobacter pyloriAbstract
The rise in antimicrobial resistance has spurred the search for plant-based alternatives due to their therapeutic properties. This study evaluates the in vitro efficacies of Datura stramonium leaves extracts against Helicobacter pylori. Leaves were extracted using selected polar and non-polar solvents, and antibacterial activity were assessed through the agar well diffusion method at varying concentrations with H.pylori as test bacteria. Phytochemical analysis was conducted using standard methods. Results showed that at 500 mg/ml, ethanolic extracts of Datura stramonium showed the highest zone of inhibition (22.02 ± 0.02 mm), followed by n-Hexane (17.03 ± 0.03 mm) and aqueous extracts (15.03 ± 0.03 mm). Ethanolic and n-Hexane of Datura stramonium extracts had high MIC values (125 mg/ml), while the aqueous extract had 250 mg/ml. Ethanolic extract and n-Hexane extracts of Datura stramonium also had bactericidal activity at 500 mg/ml, unlike the aqueous extracts. Phytochemical analysis revealed the presence glycosides (74.4± 0.04) mg/ml, phenols (131.8±0.03) mg/ml, alkaloids (100.5±0.00) mg/ml flavonoids (2.6±0.02) mg/ml, tannins (124.2±0.02) mg/ml, saponins (14.1±0.02) mg/ml, triterpenoids (37.9±0.00) mg/ml, and steroids (31.9±0.01) mg/ml in the extracts. The results showed that Datura stramonium leaf ethanolic extracts had strong antibacterial activity against H. pylori, suggesting that they could be used as an alternative to conventional therapies for H. pylori infections.
References
Ani, J. U., Akpomie, K. G., Okoro, U. C., Aneke, L. E., Onukwuli, O. D., & Ujam, O. T. (2020). Potentials of activated carbon produced from biomass materials for sequestration of dyes, heavy metals, and crude oil components from aqueous environment. Applied Water Science, 10(2), 69. https://doi.org/10.1007/s13201-020-1149-8.
Cho, H., Smith, B., Wnuk, J., Fairbrother, D., & Ball, W. (2008). Influence of surface oxides on the adsorption of naphthalene onto multiwalled carbon nanotubes. Environmental Science & Technology, 42(8), 2899-2905. https://doi.org/10.1021/es702363e.
Chulliyil, H. M., Hamdani, I. R., Ahmad, A., Al Shoaibi, A., & Chandrasekar, S. (2024). Enhanced moisture adsorption of activated carbon through surface modification. Results in Surfaces and Interfaces, 14, 100170. https://doi.org/10.1016/j.rsurfi.2023.100170.
He, J., Zhao, Y., Zhou, Y., & Wu, S. (2023). Preparation of high-performance activated carbons from hemicellulose pre-extracted residues of poplar and their application in vocs removal. Bioresources, 18(2), 2874-2896. https://doi.org/10.15376/biores.18.2.2874-2896.
Mubari, P. K., Beguerie, T., Monthioux, M., Weiss-Hortala, E., Nzihou, A., & Puech, P. (2022). The X-ray, Raman and TEM signatures of cellulose-derived carbons explained. C, 8(1), 4. https://doi.org/10.3390/c8010004.
Nabavi, E., Sabour, M., & Dezvareh, G. A. (2022). Ozone treatment and adsorption with granular activated carbon for the removal of organic compounds from agricultural soil leachates. Journal of Cleaner Production, 335, 130312. https://doi.org/10.1016/j.jclepro.2021.130312.
Nayl, A. E. A., Elkhashab, R. A., El Malah, T., Yakout, S. M., El-Khateeb, M. A., Ali, M. M. S., & Ali, H. M. (2017). Adsorption studies on the removal of COD and BOD from treated sewage using activated carbon prepared from date palm waste. Environmental Science and Pollution Research, 24(28), 22284–22293. https://doi.org/10.1007/s11356-017-9878-4.
Reza, S., Yun, C. S., Afroze, S., Radenahmad, N., AbuBakar, M. S., Saidur, R., Taweekun, J. & Azad, A. K. (2020). Preparation of activated carbon from biomass and its’applicationsin water and gas purification, a review. Arab Journal of Basic and Applied Sciences, 27(1), 208–238. https://doi.org/10.1080/25765299.2020.1766799.
Shen, F., Liu, J., Gu, C., & Wu, D. (2019). Roles of oxygen functional groups in hydrogen sulfide adsorption on activated carbon surface: a density functional study. Industrial & Engineering Chemistry Research, 58(14), 5526-5532. https://doi.org/10.1021/acs.iecr.9b00507.
Sun, Y., Li, H., Li, G., Gao, B., Yue, Q., & Li, X. (2016). Characterization and ciprofloxacin adsorption properties of activated carbons prepared from biomass wastes by H3PO4 activation. Bioresource Technology, 217, 239–244. https://doi.org/10.1016/j.biortech.2016.03.047.
Subki, N. S., Akhir, N. M., Abdul Halim, N. S., & Nik Yusoff, N. R. (2020). COD Reduction in Industrial Wastewater Using Activated Carbon Derived from Wodyetia Bifurcata Fruit. IOP Conference Series: Earth and Environmental Science, 549(1), 012066. https://doi.org/10.1088/1755-1315/549/1/012066.
Vakili, A., Zinatizadeh, A. A., Rahimi, Z., Zinadini, S., Mohammadi, P., Azizi, S., Karami, A., & Abdulgader, M. (2023). The impact of activation temperature and time on the characteristics and performance of agricultural waste-based activated carbons for removing dye and residual COD from wastewater. Journal of Cleaner Production, 382, 134899. https://doi.org/10.1016/j.jclepro.2022.134899.
Varsani, V., Vyas, S. J., & Dudhagara, D. R. (2022). Development of bio-based material from the Moringa oleifera and its bio-coagulation kinetic modeling–A sustainable approach to treat the wastewater. Heliyon, 8(9), e10447. https://doi.org/10.1016/j.heliyon.2022.e10447
Published
How to Cite
Issue
Section
FUDMA Journal of Sciences
