SCREENING OF BIOSURFACTANT-PRODUCING PSEUDOMONAS AND BACILLUS SPECIES ISOLATED FROM ENGINE OIL CONTAMINATED SOIL FOR BIODECOLORIZATION OF TEXTILE EFFLUENTS
Abstract
Textile industrial activities have been characterized in its usage of large volumes of water and variety of chemicals. Surface-active chemical compounds produced by vast varieties of microbial species are known as biosurfactant. This study aimed at screening of biosurfactant-producing Pseudomonas and Bacillus species isolated from engine oil contaminated soil for biodecolorization of textile effluents. Engine-oil polluted soil sample was collected from a mechanic work shop in Abakpa Kaduna state. Bacteria from oil polluted soil was isolated by standard spread plate technique. Physical properties of the effluent sample were determined. Screening of isolates for biosurfactant production potential was achieved using standard method. The PCR amplification of the isolates was carried out using 16S rDNA primers. The results obtained revealed that Bacillus had the highest frequency of occurrence (26.6%), followed by Micrococcus, Pseudomonas and Staphylococcus (20% each) while Acinetobacter had (13.3%). isolate S9 had the highest emulsification index (52.5%). On the other hand, isolate S11 had the lowest emulsification index (44.8%). Pseudomonas aeruginosa S5 had 99% homologue with Pseudomonas aeruginosa MSSRFV220 while Bacillus subtilis S5 had 75.59% homologue with Bacillus subtilis BC5. Textile effluent with 1.0 mg of biosurfactant from Pseudomonas aeruginosa had the highest pH value (8.0) while textile effluent with 1.0 mg of biosurfactant from Bacillus subtilis recorded the least pH value (7.6). Textile effluent amended with biosurfactant from Bacillus subtilis showed a higher temperature value (37oC) while the least temperature value was (33oC). It is concluded therefore, that Bacillus and Pseudomonas species are good biosurfactant producers.
References
Anitha, M., Kamarudin, S. K., Shamsul, N. S., & Kofli, N. T. (2015). Determination of bio_methanol as intermediate product of anaerobic co_digestion in animal and agriculture wastes. international journal of hydrogen energy, 40(35), 11791_11799. DOI: https://doi.org/10.1016/j.ijhydene.2015.06.072
Agbo, S. O., Mustapha, M. A., Ogaugwu, C. E., Sodipe, O. G., Chukwu, E. C., Raji, M. F., & Nebo, U. C. (2021). Sensitivity of Earthworm (Eisenia fetida) in Mining Soil from Ijero_Ekiti, Nigeria. Journal of Applied Sciences and Environmental Management, 25(4), 609613 DOI: https://doi.org/10.4314/jasem.v25i4.19
Bodour AA, Drees KP, Raina MM (2003) Distribution of biosurfactant_producing bacteria in undisturbed and contaminated arid Southwestern soils. Appl Environ Microbiol 69:32803287 DOI: https://doi.org/10.1128/AEM.69.6.3280-3287.2003
Bound, J.P and Voulvoulis, N., (2005). Household disposal of pharmaceuticals as a pathway for aquatic contamination in the United Kingdom, Environmental Health Perspectives, 113(12):17051711 DOI: https://doi.org/10.1289/ehp.8315
Barbusiski, K., Parzentna_Gabor, A., & Kasperczyk, D. (2021). Removal of odors (mainly H2S and NH3) using biological treatment methods. Clean Technologies, 3(1), 138155 DOI: https://doi.org/10.3390/cleantechnol3010009
Ghoreishi, S.M., and Haghighi, R., (2003). Chemical catalytic reaction and biological oxidation for treatment of non_biodegradable textile effluent, Chemical Engineering Journal,95(1):163169 DOI: https://doi.org/10.1016/S1385-8947(03)00100-1
Hassan, M. A., Noureldin, E. A., Mahmoud, M. A., & Fita, N. A. (2017). Molecular identification and epizootiology of Aeromonas veronii infection among farmed Oreochromis niloticus in Eastern Province, KSA. The Egyptian Journal of Aquatic Research, 43(2), 161167. DOI: https://doi.org/10.1016/j.ejar.2017.06.001
Hassanshahian, M., Zeynalipour, M. S., & Musa, F. H. (2014). Isolation and characterization of crude oil degrading bacteria from the Persian Gulf (Khorramshahr provenance). Marine pollution bulletin, 82(1_2), 39_44 DOI: https://doi.org/10.1016/j.marpolbul.2014.03.027
Khan, S., & Malik, A. (2014). Environmental and health effects of textile industry wastewater. Environmental Deterioration and Human Health: Natural and Anthropogenic Determinants, 5571 DOI: https://doi.org/10.1007/978-94-007-7890-0_4
Khan, S., & Malik, A. (2018). Toxicity evaluation of textile effluents and role of native soil bacterium in biodegradation of a textile dye. Environmental Science and Pollution Research, 25, 44464458 DOI: https://doi.org/10.1007/s11356-017-0783-7
Kumar, G., Kumar, R., & Sharma, A. (2015). Characterization of biosurfactants from indigenous soil bacteria recovered from oil contaminated sites. Journal of Environmental Biology, 36(5), 1101
Kumar, P. S., Joshiba, G. J., Femina, C. C., Varshini, P., Priyadharshini, S., Karthick, M. A., & Jothirani, R. (2019a). A critical review on recent developments in the low_cost adsorption of dyes from wastewater. Desalin. Water Treat, 172, 395416 DOI: https://doi.org/10.5004/dwt.2019.24613
Mahbub, K. R., Morium, B., Ahmed, M. M., Akond, M. A., & Andrews, S. (2015). Decolorization of novacron blue and novacron super black azo dyes by Bacillus spp isolated from textile effluents in Bangladesh. J Sci Res, 7(1_2), 45_53 DOI: https://doi.org/10.3329/jsr.v7i1-2.18682
Nayak, N. S., Thacker, S. C., Tipre, D. R., & Dave, S. R. (2020). Bacillus pumilusA marine bacterium: Unexplored source for potential biosurfactant production. Bioscience Biotechnology Research Communications, 13(1), 180187
Olaganathan Rajee, Patterson Jamila (2012). Biodegradation of textile dye anthraquinone vat blue 4 by Pseudomonas aeruginosa. International Journal of Advance Biotechnology and Research. 3:743_751
Obiakalaije, U. M., Makinde, O. A., & Amakoromo, E. R. (2015). Bioremediation of crude oil polluted soil using animal waste. International Journal of Environmental Bioremediation & Biodegradation, 3(3), 79_85
Pandey A, Singh P, Iyengar L (2007). Bacterial decolorization and degradation of azo dyes. Internetional Biodeterioration and Biodegradation, 59:73_84. DOI: https://doi.org/10.1016/j.ibiod.2006.08.006
Patowary, K., Patowary, R., Kalita, M. C., & Deka, S. (2016). Development of an efficient bacterial consortium for the potential remediation of hydrocarbons from contaminated sites. Frontiers in microbiology, 7, 1092 DOI: https://doi.org/10.3389/fmicb.2016.01092
Plaza, G. A., Zjawiony, I., & Banat, I. M. (2006). Use of different methods for detection of thermophilic biosurfactant producing bacteria from hydrocarbon_contaminated and bioremediated soils. Journal of Petroleum Science and Engineering, 50, 7177 DOI: https://doi.org/10.1016/j.petrol.2005.10.005
Prabha, S., Gogoi, A., Mazumder, P., Ramanathan, A. L., & Kumar, M. (2017). Assessment of the impact of textile effluents on microbial diversity in Tirupur district, Tamil Nadu. Applied Water Science, 7, 22672277 DOI: https://doi.org/10.1007/s13201-016-0394-3
Pourfadakari, S., Ghafari, S., Takdastan, A., & Jorfi, S. (2021). A salt resistant biosurfactant produced by moderately halotolerant Pseudomonas aeruginosa (AHV_KH10) and its application for bioremediation of diesel_contaminated sediment in saline environment. Biodegradation, 32, 327_341 DOI: https://doi.org/10.1007/s10532-021-09941-2
Qin J.J., Htun M. and Kekre K.A. (2007). Nanofiltration for recovering wastewater from a specific dyeing facility. Separation and Purification Technology, 56:199203. DOI: https://doi.org/10.1016/j.seppur.2007.02.002
Sani, A., Qin, W. Q., Li, J. Y., Liu, Y. F., Zhou, L., Yang, S. Z., & Mu, B. Z. (2023). Structural diversity and applications of lipopeptide biosurfactants as biocontrol agents against phytopathogens: A review. Microbiological Research, 127518 DOI: https://doi.org/10.1016/j.micres.2023.127518
Sarkar, S., Banerjee, A., Halder, U., Biswas, R., & Bandopadhyay, R. (2017). Degradation of synthetic azo dyes of textile industry: a sustainable approach using microbial enzymes. Water Conservation Science and Engineering, 2, 121131 DOI: https://doi.org/10.1007/s41101-017-0031-5
Satpute, S. K., Banat, I. M., Dhakephalkar, P. K., Banpurkar, A. G., & Chopade, B. A. (2010). Biosurfactants, bioemulsifiers and exopolysaccharides from marine microorganisms. Biotechnology advances, 28(4), 436_450. DOI: https://doi.org/10.1016/j.biotechadv.2010.02.006
Shekhar, S., Sundaramanickam, A., & Balasubramanian, T. (2015). Biosurfactant producing microbes and their potential applications: a review. Critical Reviews in Environmental Science and Technology, 45(14), 15221554 DOI: https://doi.org/10.1080/10643389.2014.955631
Singh, N. P., Sharma, J. K., & Santal, A. R. (2016). Biotechnological approaches to remediate soil and water using plantmicrobe interactions. Phytoremediation: Management of Environmental Contaminants, Volume 4, 131152. DOI: https://doi.org/10.1007/978-3-319-41811-7_8
Urum and Pekdemir T. (2004).Evaluation of biosurfactants for crude oil contaminated soil washing. Chemosphere. 57:1139_1150 DOI: https://doi.org/10.1016/j.chemosphere.2004.07.048
Wardlow, J. L., Cooray, A., Osage, W., Bourne, N., Clements, D., Dannerbauer, H., ... & Verma, A. (2017). The Interstellar Medium in High_redshift Submillimeter Galaxies as Probed by Infrared Spectroscopy. The Astrophysical Journal, 837(1), 12 DOI: https://doi.org/10.3847/1538-4357/837/1/12
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