EXPLORING THE EFFECTIVENESS OF Pseudomonas aeruginosa ISOLATES FOR BIOREMEDIATION OF CRUDE OIL-CONTAMINATED SOILS USING SOYBEAN HULL AS A BIOSTIMULANT: A FOCUS ON ETPH AND PAHs
DOI:
https://doi.org/10.33003/fjs-2024-0804-2575Keywords:
Bioremediation, Indigenous bacteria, Crude oil degradation, Soil pollution, Biosurfactant productionAbstract
The Speciated EPA-16 Polyaromatic Hydrocarbons (PAHs) in crude oil pose significant environmental and health risks due to their harmful properties. This study focused on indigenous bacteria capable of degrading Extractable Total Petroleum Hydrocarbons (ETPHs) and PAHs through a Nutrient-Amended Bioaugmentation Strategy (N-ABS). Crude oil-degrading bacteria were isolated from contaminated soil in Alakiri Community, Rivers State, Nigeria, leading to the identification of four Pseudomonas aeruginosa isolates (KUD1-4) via 16S rRNA gene sequencing. Bioremediation treatments, using both bacterial isolates and Soybean hull as a biostimulant, showed that P. aeruginosa KUD2 achieved the highest removal efficiencies of 96.59% for ETPHs and 80.00% for PAHs. This study underscores the potential of P. aeruginosa KUD2 and Soybean hull in remediating crude oil-contaminated soils.
References
Alcock R..P., Huynh W., Chalil, R. & Andrew, G.M. (2023). CARD 2023 : Expanded curation, support for machine learning and resistome prediction at the comprehensive antibiotic resistance database. Nucleic Acids Research, 51(1), 690- 699.
Alexander, S., Zemphira, A. & Glenn, M (2001). Bacteriophage therapy. Antimicrobial agents and Chemotherapy, 45 (3), 649-659.
Amdt, D., Grant, J., Marcu, A., Sajed, T., Pon, A., Liang, Y. & Wishart, D.S. (2016). PHASTER : A better, faster version of the PHAST phage search tool. Nucleic Acids Research, 44 (1) ,16-21.
Domingo, C.P. & Delgado, J. (2018). Bacteriophages protagonists of a post antibiotic era. Antibiotics, 7 , 55-66.
Furtoro, L.L., Pyne, S.P. & Cheng, B.J. (2018). Bacteriophage therapy : Clinical trials and regulatory hurdles. Frontiers in cellular and infection microbiology, 8 : 376
Gorski, A., Wazna, E., Dabrowsi,B., Dabrowskki, K. & Miedzybrodzki, R (2006). Bacteriophage translocation. Immunological and Medicinal Microbiology, 46, 313-319.
Kakasis, A and Panitsa, G (2019). Bacteriophage therapy as an alternative treatment for human infections. International Journal of Antimicrobial Agents, 53(1), 16-21.
Minasyan, H.(2017). Sepsis and septic shock pathogenesis and treatment perspectives. Journal of Critical Care , 40, 229-42.
Olson, R.D., Assaf, R., Brettin, T & Stevens, R.L. (2023). Introducing the bacterial and viral bioinformatic resource centre. Nucleic Acids Research, 51 (1) , 678-689.
O" Neill, J.(2016). Tackling drug resistant infections globally: Final report and recommendations. The Review of Antimicrobial resistance.
Pouilot, F., Chomtom, M., Bloi, H., Courroux, C. & Bidet, P. (2012). Efficacy of bacteriophage Therapy in experimental sepsis and meningitis caused by a clone Escherichia coli strain. Antimicrobial agents and Chemotherapy, 56 , 3568-3575.
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