POOR PERFORMANCE OF A POPULAR MALARIA RAPID DIAGNOSTIC KIT COMPARED TO MICROSCOPY IN ADULTS P. FALCIPARUM INFECTIONS IN NIGERIA

Authors

  • Oluwaremilekun Grace Ajakaye Adekunle Ajasin University, Akungba Akoko

DOI:

https://doi.org/10.33003/fjs-2024-0803-2456

Keywords:

Malaria, RDTs, Microscopy, Adults, Parasite density, Nigeria

Abstract

Rapid diagnostic tests (RDTs) have become the mainstay of malaria diagnosis, especially in endemic rural settings where microscopy is difficult to perform. However, the performance of malaria rapid diagnostic tests is infrequently accessed in adults in Nigeria. We therefore accessed the performance of CareStartTM malaria RDT in the routine detection of Plasmodium in adults. A total of 134 participants were tested for malaria infection using both microscopy and CareStartTM Malaria Pf (HRP2) Ag RDT (ACCESSBIO, USA) in Owo, Ondo State, Nigeria. The prevalence of malaria was higher based on microscopy (20.1%) compared to RDT (3.7%), with a mean parasite density of 2954.1 parasites per microliter of blood. The sensitivity and specificity of the RDT were 3.70% and 96.26%, respectively, while the positive predictive value and negative predictive value were 20.00% and 79.84%, respectively. Although RDT sensitivity varied significantly with parasite density, it failed to detect malaria infection with high parasitaemia. Our observation suggests possible reduced diagnostic sensitivity of RDT for the diagnosis of P. falciparum malaria in adults and the need to support the interpretation of RDT results with microscopy.

References

Abdullah, A. and Rehbein, H. (2017). DNA barcoding for the species identification of commercially important fishery products in Indonesian markets. International Journal of Food Science and Technology. 52(1): 266-274. https://doi.org/10.1111/ijfs.13278

Bingpeng, X., Heshan, L., Zhilan, Z., Chunguang, W., Yanguo, W. and Jianjun, W. (2018). DNA barcoding for identification of fish species in the Taiwan Strait. PloS one, 13(6) p.e0198109. https://doi.org/10.1371/journal.pone.0198109

Bolaji, D.A., Lawal-Are, A.O. and Kuton, M.P. (2023). DNA barcoding and misidentification of some marine fish species in Nigerian industrial trawl fishery. Scientific African, 20, e01662. https://doi.org/10.1016/j.sciaf.2023.e01662

Carpenter, K.E. and De Angelis, N. (2016). The living marine resources of the Eastern Central Atlantic (Vol 4 Bony fishes part 2 (Perciformes to Tetradontiformes) and Sea turtles. Rome: Food and agriculture organization of the United Nations. 2351 – 3132 pages. https://www.fao.org/documents/card/en?details=2105cb6a-b95f-4389-8836-af45a6b5ae9d

DeSalle, R. and Goldstein, P. (2019). Review and interpretation of trends in DNA barcoding. Frontiers in Ecology and Evolution. 7, 302. https://doi.org/10.3389/fevo.2019.00302.

Falade, M. O., Opene, A. J., & Benson, O. (2016). DNA barcoding of Clarias gariepinus, Coptodon zillii and Sarotherodon melanotheron from Southwestern Nigeria. F1000Research, 5. doi: 10.12688/f1000research.7895.1

Hebert, P.D., Cywinska, A., Ball, S.L. and DeWaard, J.R. (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences. 270: 313-321. DOI: 10.1098/rspb.2002.2218

Hubert, N., Hanner, R., Holm, E., Mandrak, N.E., Taylor, E., Burridge, M. and Bernatchez, L. (2008). Identifying Canadian freshwater fishes through DNA barcodes. PLoS one. 3(6), e2490. https://doi.org/10.1371/journal.pone.0002490

Published

2024-06-30

How to Cite

Ajakaye, O. G. (2024). POOR PERFORMANCE OF A POPULAR MALARIA RAPID DIAGNOSTIC KIT COMPARED TO MICROSCOPY IN ADULTS P. FALCIPARUM INFECTIONS IN NIGERIA. FUDMA JOURNAL OF SCIENCES, 8(3), 390 - 394. https://doi.org/10.33003/fjs-2024-0803-2456