MITIGATION OF COPPER-INDUCED TOXICITY IN WISTAR RATS' SERUM BIOCHEMICAL PROFILE BY CANNABIS LEAF EXTRACT

Authors

  • Ashiru Dahiru
    Department of Veterinary Physiology and Biochemistry, Usmanu Danfodiyo University Sokoto, Nigeria
  • Hassan Maina Ibrahim
    Department of Veterinary Physiology and Biochemistry, Usmanu Danfodiyo University Sokoto, Nigeria
  • Bashir Saidu
    Department of Veterinary Physiology and Biochemistry, Usmanu Danfodiyo University Sokoto, Nigeria
  • Nafisat Abdulazeez
    Department of Veterinary Physiology and Biochemistry, Usmanu Danfodiyo University Sokoto, Nigeria
  • Muhammad Mahuta Muawiyyah
    Department of Veterinary Theriogenology, Federal University of Agriculture Zuru, Kebbi State
  • Sulaiman Sunusi
    Department of Veterinary Physiology and Biochemistry, Usmanu Danfodiyo University Sokoto, Nigeria
  • Ishaq Abdullahi Jaafaru
    Department of Veterinary Physiology and Biochemistry, Usmanu Danfodiyo University, Sokoto

Keywords:

Cannabis, Copper, Serum Biochemistry, Wistar Rat

Abstract

Copper serves as an integral part of specialized cuproproteins responsible for normal growth and development of the body, and excess accumulation in the body has a detrimental effect. Anthropogenic influences and an increased number of vehicles coupled with intensive urban and rural infrastructure result in the accumulation of heavy metals in the soil and plants, which are subsequently transmitted to humans via the food chain. The study was designed to provide positive effects of cannabis, establish a means of preventing copper exposure, and devise a means of preventing them. The aim of the study was to determine the therapeutic effect of cannabis leaf extract on copper-induced toxicity and serum biochemical profile changes. The experimental study was carried out using 20 Wistar rats allocated into 4 groups containing 5 rats each: group A (negative control), group B (copper only), group C (cannabis only), and group D (copper then treated with cannabis). The results showed decreased serum electrolytes (urea, bicarbonate, creatinine, total protein, albumin, bilirubin, AST, and ALP) and increased serum ALT and erythropoietin, which were later reversed after treatment with cannabis. In conclusion, cannabis leaf extract has therapeutic effects on copper toxicity.

Dimensions

Ashton, C. H. (2001). Pharmacology and effects of cannabis: A brief review. British Journal of Psychiatry, 178(FEB.), 101106. https://doi.org/10.1192/bjp.178.2.101

Bremner, I. (1998). Manifestations of copper excess. American Journal of Clinical Nutrition, 67(5 SUPPL.). https://doi.org/10.1093/ajcn/67.5.1069S

Buck, W. B., and Sharma, R. M. (1969). Copper Toxicity in Sheep Copper Toxicity in Sheep. Iowa State University Veterinarian, 31(1). https://lib.dr.iastate.edu/iowastate_veterinarian/vol31/iss1/1

Dryburgh, L. M., Bolan, N. S., Grof, C. P. L., Galettis, P., Schneider, J., Lucas, C. J., and Martin, J. H. (2018). Cannabis contaminants: sources, distribution, human toxicity and pharmacologic effects. British Journal of Clinical Pharmacology, 84(11), 24682476. https://doi.org/10.1111/bcp.13695

Fergusson, D. M., Norwood, L. J., and Beautrais, A. L. (2003). Cannabis and educational achievement. Addiction, 98(12), 16811692. https://doi.org/10.1111/j.1360-0443.2003.00573.x

Hall, W., and Solowij, N. (1998). Adverse Effects Cannabis. The Lancet 352(9140), 16111616.

Hrapkiewicz, K., Colby, L. A., and Denison, P. (2013). Clinical laboratory animal medicine: an introduction. John Wiley and Sons

ICT Directorate, Sokoto State Government (2023). https://sokotostate.gov.ng/history-of-sokoto/the-land/.

Lewis, M. M., Yang, Y., Wasilewski, E., Clarke, H. A., and Kotra, L. P. (2017). Chemical Profiling of Medical Cannabis Extracts. ACS omega, 2(9), 6091-6103 https://doi.org/10.1021/acsomega.7b00996

Mendelson, J. H., Babor, T. F., Kuehnle, J.C., Rossi, A. M., Bernstein, J. G., Mello,N. K., and Greenberg, I. (1976). Behavioral and biological aspects of marijuana use. Annals of the New York Academy Sciences, 282(1),186-210.

Mladenovic, J., and Paunovic, M. (2014). Copper-induced changes of lipid peroxidation and hemato-biochemical parameters in rat blood: Protective role of flavonoids. Archives of Biological Sciences, 66(3), 1271-1279. https://doi.org/10.2298/ABS1403271M

Mohammed, S. A. (2014). Toxicological effect of copper sulphate and cobalt chloride as feed additives on fertility in male albino rats. Benha veterinary medical journal, 27(1), 135145.

Musacco-sebio, R., Saporito-magri, C., and Acosta, J. M. (2017). Iron and Copper Toxicity in Rat Liver: A Kinetic and Holistic Overview. 913. https://doi.org/10.17140/LROJ-2-110

Nederbragt, H., van den Ingh, T. S., and Wensvoort, P. (1984). Pathobiology of copper toxicity. The Veterinary Quarterly, 6(4). https://doi.org/10.1080/01652176.1984.9693935

Osadolor, H. B., and Mathias, A. (2010). Effects of marijuana on sodium and potassium ( na + & p + ) ions homeostasis among smokers in Benin City- a metropolitan city in Nigeria. International Journal of Pharmarceutical and Biomedical Sciences.

Osredkar, J., and Sustar, N. (2011). Copper and Zinc , Biological Role and Significance of Copper / Zinc Imbalance. Journal of Clinical Toxicology. 118. https://doi.org/10.4172/2161-0495.S3-001

Reis, L. S. L. de S., Pardo, P. E., Camargos, A. S., and Oba, E. (2010). Mineral element and heavy metal poisoning in animals. Journal of Medicine and Medical Sciences, 1(December), 560579.

Tambuwal, F. M., Shittu, A., and Abubakar, M. B. (2009). A survey of veterinary hospitals in Nigeria for the presence of some bacterial organisms of nosocomial and zoonotic potential. Veterinaria italia 45(2), 235241.

Tang, H., Xu, M., Luo, J., Zhao, L., Ye, G., Shi, F., Lv, C., Chen, H., Wang, Y., and Li, Y. (2019). Liver toxicity assessments in rats following sub chronic oral exposure to copper nanoparticles. Environmental Sciences Europe. https://doi.org/10.1186/s12302-019-0214-0

Tanko, Y., Ismail, A. S., Mohammed, K. A., Eze, E. D., Jimoh, A., Sada, N. M., Muhammad, A., and Mohammed, A. (2013). Ameliorative Effects of Magnesium and Copper Sulphates on Blood glucose and Serum Electrolytes Levels in Fructose-induced Diabetic Wistar Rats. Journal of Applied Pharmaceutical Science 3(07), 160163. https://doi.org/10.7324/JAPS.2013.3730

Published

31-03-2025

How to Cite

MITIGATION OF COPPER-INDUCED TOXICITY IN WISTAR RATS’ SERUM BIOCHEMICAL PROFILE BY CANNABIS LEAF EXTRACT. (2025). FUDMA JOURNAL OF SCIENCES, 9(3), 354-357. https://doi.org/10.33003/fjs-2025-0903-2315

Issue

Vol. 9 No. 3 (2025): FUDMA Journal of Sciences - Vol. 9 No. 3

Section

Research Articles
Copyright & Licensing

FUDMA Journal of Sciences

How to Cite

MITIGATION OF COPPER-INDUCED TOXICITY IN WISTAR RATS’ SERUM BIOCHEMICAL PROFILE BY CANNABIS LEAF EXTRACT. (2025). FUDMA JOURNAL OF SCIENCES, 9(3), 354-357. https://doi.org/10.33003/fjs-2025-0903-2315

Most read articles by the same author(s)