PROXIMATE, PHYTOCHEMICAL, MINERAL COMPOSITION AND ANTIOXIDANT EFFECTS OF METHANOL EXTRACT OF Persea americana (Avacado pear) SEED
Abstract
The proximate, phytochemical, mineral, and antioxidant composition of Persea americana seed methanol extract was examined in this work. Standard procedures were used for both proximate, mineral composition and quantitative evaluations. Lipid peroxidation, reducing power, and 2,2-diphenyl-1-picryhydrazyl tests were used to measure the antioxidant activity. Using absorption spectroscopy techniques, antioxidant vitamins A, E, and C as well as enzymes (superoxide dismutase, peroxidase, and catalase) were evaluated. Based on the proximate analysis, the P. americana seeds have higher levels of protein (21.00 ± 0.88 %), fat (25.73 ± 2.01 %), and carbohydrates (32.05 ± 1.21 %), but they have lower levels of moisture (8.98±0.10 %) and ash (3.28±0.16 %). A quantitative phytochemical study found that there were phenols (3.51±0.42 mg/100 g),saponins (0.05±0.38 mg/100 g), alkaloid (0.70±0.37mg/100 g), beta-carotene (32.24±0.55mg/100 g), lycopene (6.84±0.52 mg/100 g), flavonoids (34.14±3.13 mg/100 g), phytate (0.00±0.03 mg/100 g), cardiac glycosides (0.37±0.01 %), total phytosterol (21.25±0.12 mg/100 g), stigmasterol (13.16±0.15 mg/100 g) and campesterol (6.16±0.12 mg/100 g). The mineral composition showed that iron and manganese had the lowest quantities, while potassium, calcium, and magnesium had the highest concentrations. The analyzed samples' potential therapeutic benefits in phytomedicine may be attributed to their respective chemical compositions. Out of all the antioxidant vitamins tested, vitamin E had the highest amount (267.73 ± 0.07 mg/100g), followed by vitamin A (7.60 ± 0.01 mg/100 g) and vitamin C (3.88 ± 0.03 mg/100 g). As extract concentration increased, there was a substantial (p<0.05) rise in the DPPH scavenging effect and suppression of...
References
Adeboye, J.O., Fajonyomi, M.O., Makinde, J.M., & Taiwo, O.B. (1999). A preliminary study on the hypotensive activity of Persea americana leaf extracts in anaesthetized normotensive rats. Fitoterapia. 70(1): 15-20. DOI: https://doi.org/10.1016/S0367-326X(98)00015-X
Ajayi, I.A., & Adesanwo, O. (2009). Comparative study of the mineral element and fatty acid composition of Dacryodes edulis pulp and seed. World Journal of Agricultural Sciences. 5: 279-283.
American Public Health Association (APHA). (1995). Standard Methods for the Examination of Water and Waste Water, 19th edition APHA, Washington, D.C. 1644.
Anaka, O.N., Ozolua, R.I., & Okpo, S.O. (2009). Effect of the aqueous seed extract of Persea americana Mill (Lauraceae) on the blood pressure of Sprague Dawley rats. African Journal Pharmacy and Pharmacology.3(10): 485-490.
Antia, B.S., Akpan, E.J., Okon, P.A., & Umoren, I.U. (2006). Nutritive and anti-nutritive evaluation of sweet potatoes leaves. Pakistan Journal of Nutrition. 5(2): 166-170 DOI: https://doi.org/10.3923/pjn.2006.166.168
Arukwe, U., Amadi, B.A., Duru, M.K.C., Agomuo, E.N., Adindu, E.A., Odika, P.C., Lele, K.C., Egejuru, L., and Anudike, J. (2012). Chemical Composition of Persea americana leaf, fruit and seed. International Journal of Research and Reviews in Applied Sciences. 11(2): 2-10.
Barros, L., Falcâo, S., Baptista, P., Freire, C., Vilas-Boas, M. & Ferreira, I.C.F.R. (2008). Antioxidant activity of Agaricussp. Mushrooms by chemical, bio- chemical and electrochemical assays. Food Chemistry. 111: 61. DOI: https://doi.org/10.1016/j.foodchem.2008.03.033
Barros, L., Ferreira, M. J., Queiros, B., Ferreira, I.C.F.R & Batista, P., (2007).Total phenols, ascorbic acid, á-carotene and lycopene in portuguese wild edible mushrooms and their antioxidant activities, Food Chemistry. 103: 413-419. DOI: https://doi.org/10.1016/j.foodchem.2006.07.038
Bertling, I., Tesfay S.Z. & Bower, J.P. (2007). Antioxidant in Hass Avocado. In: South African Avocado Grower Association. 17-19.
Chikezie, P.C., Agomuo, E.N., & Amadi, B.A. (2008). Proximate analysis In: Biochemistry Practical/Research method. A Fundamental Approach. Megasoft Publishers, Owerri, 2:8-21.
Deepti, D., Rachel, M.S., Gregory, R.Z. & Joshua, D.L. (2013). Avocado (Persea americana) seed as a source of bioactive phytochemicals. Current Pharmaceutical Design. 19: 6133-6140. DOI: https://doi.org/10.2174/1381612811319340007
Dillard, C. J. and German, E. M. (1990). Coconut: In support of good health in the 21st century. 127.
Duke, J. (1992). Handbook of biological active phytochemicals and their activities. CRC Press, Boca Raton (Florida), 99-131.
Ebrahimzadem, M A., Seyed, M.N., Seyed, F.N., Fatemeh B., & Ahmad, R.B. (2009). Antioxidant and free radical scavenging activity of H. officinalis, L.angustifolius, V. odorata, B. hyrcana and C. speciosum.Pakistan Journal of Pharmaceutical Sciences.23(1): 29-34.
El-Agamey, A., Lowe, G.M., McGarvey, D.J., Mortensen, A., Phillip, D.M.,& Truscott, T.G. (2004). Carotenoid radical chemistry and antioxidant/pro-oxidant properties. Archives of Biochemistry and Biophysics. 430: 37-48. DOI: https://doi.org/10.1016/j.abb.2004.03.007
Evans, W.C. (2005). Traditional plant medicines as a source of new drugs. In Trease and Evans Pharmacognosy, 15th edition. Publishers, Elsevier. 125-134.
Gordon, M.H. (1990). The mechanism of antioxidant action in in-vitro. In : BJF Hudson Edition, Food antioxidants Elsevier Applied Science, London. DOI: https://doi.org/10.1007/978-94-009-0753-9_1
Harborne, J.B. (1973). Phytochemical methods. A guide to modern techniques of plants analysis. 2nd Edition, Chapman and Hall, Ltd., London, UK. 149-188.
Hirota, Y., Nagao, T., Watanabe, Y., Suenaga, M., Nakai, S., Kitano, M., Sugihara, A. & Shimada, Y. (2003). Purification of steryl esters from soybean oil deodorizer distillate. Journal of the American Oil Chemists' Society.80: 341-346. DOI: https://doi.org/10.1007/s11746-003-0700-6
Hurrel, R. F., Jullert, M. B., Reddy, S. R., Lynch, S. D. & Cook, J. D. (1992). Changes in anti-nutrients contents of edible vegetables under varied temperature and heating time. American Journal of Clinical Nutrition. 56: 573.
Ifesan, B.O.T., Olorunsola, B.O., & Ifesan, B.T. (2015). Nutritional composition and acceptability of candy from avocado seed (Persea americana). International Journal of Agriculture Innovations and Researches. 3(6): 2319-1473.
Imafidon, K.E.& Amaechina, F.C. (2010). Effects of aqueous seed extract of Persea americana Mill. (Avocado) on blood pressure and lipid profile in hypertensive rats. Advance Biological Researches.4(2): 116-121.
Ishida, H., Suzunoh, S., Sugiyana, N., Innami, S., Todoro, T. & Mackawa, A. (2000). Nutritional evaluation of chemical components of leaves, stalk and stem of sweet potatoes (Ipomea botatus). Food Chemistry. 68: 359-367. DOI: https://doi.org/10.1016/S0308-8146(99)00206-X
João, J.L., Érika, H.B., Rossana, A.C., Raimunda, S.N., José, J.C., Luciana, M.B., Selene, M.M. & Marcos, G.R. (2009). Chemical composition, toxicity and larvicidal and antifungal activities of Persea americana (avocado) seed extracts. Revista da Sociedade Brasileira de Medicina Tropical. 42(2):110-113. DOI: https://doi.org/10.1590/S0037-86822009000200003
Justina, Y. T., Olukemi, A.O., Ajayi, O.O. & Adegoke, G.O. (2016). Nutritional and antinutritional compositions of processed Avocado (Persea americana Mill) seeds.Journal of Plant Science and Research. 6(2):6-12.
Klein, B.P. and Perry, A.K. (1982). Ascorbic acid and vitamin A activity in selected vegetables from different geographical areas of the United States. Journal of Food Science. 47: 941-945. DOI: https://doi.org/10.1111/j.1365-2621.1982.tb12750.x
Kopp, L. E. (1966). A taxonomic revision of the genus Persea in the western hemisphere. (Perseae-Lauraceae). Memoirs of The New York Botanical Garden. 14(1):1-117.
Krogdahl, A.& Bakk-Mckellep, A.M. (2002). Physiological mechanism in the impairment of nutrient digestion and transport capacity in salmon intestine. Soybean Association Hudson Iowa, USA. 128.
Kumar, A.K., Ramachandra, S.S.& Narsu, L. (2010). Pharmacognostic and Phytochemical investigation of roots of Hibiscus micranthus Linn. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 1(4): 324-337.
Larissa, B. D C.A., Sarah, L.S., Marcos, A.M.G, Magda, R.A.F, Evani, L.A., Karina, P.R. & Luiz, A.L. (2013). Total phytosterol content in drug materials & extracts from roots of Acanthospermum hispidum by UV-VIS spectrophotometry. Brazilian Journal of Pharmacognosis.23: 736-742. DOI: https://doi.org/10.1590/S0102-695X2013000500004
Larrauri, J.A., Goni, I., Martin-Carron, N., Ruperez P. & Saura-Calixto, F. (1996). Measurement of health-promoting properties in fruit dietary fibes: antioxidant capacity, fermentability and glucose retardation index. Journal of the Science of Food and Agriculture. 71:515-519. DOI: https://doi.org/10.1002/(SICI)1097-0010(199608)71:4<515::AID-JSFA611>3.3.CO;2-P
Liener, I. E. (1994). Implications of antinutritional components in soybean foods. Critical Reviews in Food Science and Nutrition. 34(1):31-67. DOI: https://doi.org/10.1080/10408399409527649
Merrill, A.L. & Watt, B.K. (1973). Energy value of foods: basis and derivation. Agriculture Handbook No. 74. Washington, DC, ARS United State Department of Agriculture.
Mohammad, A., Koji, Y., Toshiki, M., Yomi, N., Katsumi, S., Hiroaki, S. & Yoshifumi, T. (2007). Superoxide anion radical scavenging activities of herbs and pastures in northern Japan determined using electron spin resonance spectrometry. International Journal of Biological Sciences. 3:349-355. DOI: https://doi.org/10.7150/ijbs.3.349
Morton, J. F. and Dowling, C.F. (1987). Avocado in: Fruits of Warm Climate. Winterville, N.C. 91:102
Okorie, N.H Umerie,S.C., Emeka, I. C, Ali I.J., (2023). in-vitro anti-inflammatory evaluation of methanol and butanol extracts of avoacado pear (persea americana mill) fruit peel. African Journal of Pharmaceutical research and development, 15(3),1-6 DOI: https://doi.org/10.59493/ajopred/2023.3.1
Niizuma, H., Nakamura, Y., Ozaki, T., Nakanishi, H. & Ohira, M. (2006). Bcl-2 is a key regulator for the retinoic acid-induced apoptotic cell death in neuroblastoma. Oncogene. 25: 5046-5055. DOI: https://doi.org/10.1038/sj.onc.1209515
Nwaoguikpe, R . N. & Braide, W. (2011). The effect of aqueous seed extract of Persea americana (avocado pear) on serum lipid and cholesterol levels in rabbits.African Journal of Pharmacy and Pharmacology Research. 1(2): 023-029.
Okwu, D. E. & Okwu, M. E. (2004). Chemical composition of Spondiasmombia Linn plant parts. Journal of Sustainable Agriculture and the Environment.6:140-147.
Okwu, D.E, (2004). Phytochemicals and vitamin content of indigenous spices of South Eastern Nigeria. Journal 0f Sustainable Agriculture and Environment. 6: 30-34.
Olusanya, J. O. (2008). Proteins, In: Essentials of food and nutrition. Apex Books Limited, Lagos. 13-21.
Onuegbu, N.C., Nwuka, M.U., Ojukwu, M. & Kabu, N.O. (2016). Nutritional properties of African pear seed and performance of defatted cake in poultry feed formulations. AnimalNutrition. 1(29):1-3.
Adeniyi, S.A., Orjiekwe, C.L. and Ehiagbonare, J.E. (2009). Determination of alkaloids and oxalates in some selected food samples in Nigeria. African Journal of Biotechnology. 8(1): 110-112.
Oyaizu, M. (1986). Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Japanese Journal of Nutrition. 44: 307–315. DOI: https://doi.org/10.5264/eiyogakuzashi.44.307
Passmore, R. & Eastwood, M.A. (1986). In Davison Passmore, R., Human Nutrition and Dietetics, Churchill Livingstone, London, 124-126.
Pearson, D. (1974). The Chemical Analysis of Foods. Churchhill Livingstone, Edinburgh. 6th Edition. 457.
Ramos, M.R., Jerz, G., Villanueva, S., Lopez-Dellamary, F., Waibe, R. & Winterhalter, P. (2004). Two glucosylated abscisic acid derivates from avocado seeds (Persea americana Mill. Lauraceae cv. Hass). Phytochemistry. 65: 955-962. DOI: https://doi.org/10.1016/j.phytochem.2003.12.007
Reddy, K.P., Subhani, S.M., Khan, P.A. & Kumar, K.B. (1995). Effect of light and benzyl adenine and dark-treated graving rice (Oryza sativa) leaves –changes in peroxidases activity. Plant Cell Physiology.26: 987-994. DOI: https://doi.org/10.1093/oxfordjournals.pcp.a077018
Rutkowski, M., Grzegorczyk, K. & Paradowski M.T. (2005). Colorimetric method of blood plasma total vitamin E determination – the own modification of Tsen method]. Diagnostic Laboratory.41: 375.
Rutkowski, M., Grzegorczyk, K., Gendek, E. & Kedziora, J. (2006). Laboratory convenient modification of Bessey method for vitamin A determination in blood plasma. Journal of Physiological Pharmacy. 57: 221.
Salah, W., Miller, N.J., Pangauga, T., Bolwell, G.P., Rice, E., & Evans, C. (1995). Polyphenolic flavonols as scavengers of aqueous phase radicals as chainbreaking antioxidant.Archives of Biochemistry and Biophysics. 2:339-346. DOI: https://doi.org/10.1006/abbi.1995.1473
Scandalios, J.G. (1993). Oxygen stress and superoxide dismutases. Plant Physiology. 101: 7-12. DOI: https://doi.org/10.1104/pp.101.1.7
Shimada, K., Fujikawa, K., Yahara, K., & Nakamura, T. (1992). Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of Agriculture and Food Chemistry.40: 945–948. DOI: https://doi.org/10.1021/jf00018a005
Sodipo, O.A., & Akiniyi, J. (2000). Studies on certain characteristics of extracts from bark of Pansinystaliamacruceras (K. schum). Pierre Exbeille. Global Journal of Pure and Applied Science. 6:83-87. DOI: https://doi.org/10.4314/gjpas.v6i1.16082
Stray, F. (1988). The national guide to medicinal herbs and plants. Tiger Books International, London. 1246.
Sun, M. & Zigma, S. (1978). An improved spectrophotometer assay of superoxide dismutase based on epinephrine antioxidation. Analalytic Biochemistry. 90: 81-89. DOI: https://doi.org/10.1016/0003-2697(78)90010-6
Susan, D.A. (1998), "Vitamin A", Kirk-Othmer Encyclopedia of Chemical Technology (45), New York: John Wiley, pp. 99–107.
Trease, G.E. & Evans, W.C. (1989). Phytochemicals. Pharmoscognosy. 13th ed. ELBS/Bailliere Tindal, London. pp 345–346.
Umerie, C.S., Okorie N H., Dike D.U., & Ani, I.N (2022). Phytochemical Analysis and Antioxidant Activity of Avocado Pear Peel (Persea americana) Extract. Journal of Pharmaceutical Research. International. 34(28A): 22-29. DOI: https://doi.org/10.9734/jpri/2022/v34i28A36018
Usoh, F.I., Akpan, E.J., Etim, E.O. & Farombi, E.O. (2005). Antioxidant actions of dried flower of Hibiscus sabidariffa L. on sodium arsenite-induced oxidative stress. Pakistan Journal of Nutrition. 4:135-141. DOI: https://doi.org/10.3923/pjn.2005.135.141
Young, S. M. & Greaves, J. S. (1940). Influence of variety and treatment on phytic acid content of wheat. Journal of Food Research. 5:103-105 DOI: https://doi.org/10.1111/j.1365-2621.1940.tb17171.x
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