• Ochuko Joshua Eriegha Nigeria Maritime University, Okerenkoko
  • Jerimoth Kesena Ekelemu
  • Oster Francis Nwachi
Keywords: Biodiversity, Mitochondrial DNA, Phylogenetic diversity, cytochrome c oxidase I, Jackfish


Fish species show astonishing phenotypic and genetic diversity in populations. The delimitation and recognition of fish species are not only of interest to taxonomists but also a requirement in studies of ecology. In this study, the genetic structure of Caranx senegallus collected from three water bodies adjoining the Gulf of Guinea, Nigeria was characterized using the cytochrome c oxidase I (COI) barcode gene region. Fish samples collected from Qua Iboe estuary, Escravos estuary, and Lagos lagoon were identified morphologically, and muscle tissues were used for DNA barcoding using Fish1 and Fish2 primers. Evolutionary analysis showed the formation of diverging clades in the neighbor-joining tree and indicated the presence of multiple species. The result also revealed that morphological identification was not entirely successful, as the Basic Local Alignment Search Tool (BLAST) prediction confirmed the presence of three specimens initially identified as C. senegallus but genetically matching C. fischeri and Trachinotus goreensis. This observation further emphasized the need for an integrative method for species identification.


Abdullah, A. and Rehbein, H. (2017). DNA barcoding for the species identification of commercially important fishery products in Indonesian markets. International Journal of Food Science and Technology. 52(1): 266-274. https://doi.org/10.1111/ijfs.13278 DOI: https://doi.org/10.1111/ijfs.13278

Bingpeng, X., Heshan, L., Zhilan, Z., Chunguang, W., Yanguo, W. and Jianjun, W. (2018). DNA barcoding for identification of fish species in the Taiwan Strait. PloS one, 13(6) p.e0198109. https://doi.org/10.1371/journal.pone.0198109 DOI: https://doi.org/10.1371/journal.pone.0198109

Bolaji, D.A., Lawal-Are, A.O. and Kuton, M.P. (2023). DNA barcoding and misidentification of some marine fish species in Nigerian industrial trawl fishery. Scientific African, 20, e01662. https://doi.org/10.1016/j.sciaf.2023.e01662 DOI: https://doi.org/10.1016/j.sciaf.2023.e01662

Carpenter, K.E. and De Angelis, N. (2016). The living marine resources of the Eastern Central Atlantic (Vol 4 Bony fishes part 2 (Perciformes to Tetradontiformes) and Sea turtles. Rome: Food and agriculture organization of the United Nations. 2351 – 3132 pages. https://www.fao.org/documents/card/en?details=2105cb6a-b95f-4389-8836-af45a6b5ae9d

DeSalle, R. and Goldstein, P. (2019). Review and interpretation of trends in DNA barcoding. Frontiers in Ecology and Evolution. 7, 302. https://doi.org/10.3389/fevo.2019.00302. DOI: https://doi.org/10.3389/fevo.2019.00302

Falade, M. O., Opene, A. J., & Benson, O. (2016). DNA barcoding of Clarias gariepinus, Coptodon zillii and Sarotherodon melanotheron from Southwestern Nigeria. F1000Research, 5. doi: 10.12688/f1000research.7895.1 DOI: https://doi.org/10.12688/f1000research.7895.1

Hebert, P.D., Cywinska, A., Ball, S.L. and DeWaard, J.R. (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences. 270: 313-321. DOI: 10.1098/rspb.2002.2218 DOI: https://doi.org/10.1098/rspb.2002.2218

Hubert, N., Hanner, R., Holm, E., Mandrak, N.E., Taylor, E., Burridge, M. and Bernatchez, L. (2008). Identifying Canadian freshwater fishes through DNA barcodes. PLoS one. 3(6), e2490. https://doi.org/10.1371/journal.pone.0002490 DOI: https://doi.org/10.1371/journal.pone.0002490

How to Cite
ErieghaO. J., EkelemuJ. K., & NwachiO. F. (2024). DNA BARCODING OF Caranx senegallus FROM THREE WATER BODIES ADJOINING THE GULF OF GUINEA, NIGERIA. FUDMA JOURNAL OF SCIENCES, 8(1), 293 - 298. https://doi.org/10.33003/fjs-2024-0801-2242