INCIDENCE OF Listeria monocytogenes FROM DIFFERENT FOOD SAMPLES IN YOLA
Abstract
Listeria monocytogenes causes food-borne listeriosis in humans. Vegetables and animal-based foods facilitate transmission and contamination if mishandled. The research aims to isolate and identify L. monocytogenes from different food samples in the study area. The Food and Drug Administration, Bacteriology and Analytical Methods (FDA, BAM) were utilized to isolate L. monocytogenes. A total of 120 food samples were examined, consisting of 10 samples each of cabbage and fruit salads, as well as 20 samples each of fresh fish, meat, frozen chicken, ice cream, and yoghurt. These samples were purchased at random from various shops and markets in Yola at different times. The identification of isolates was performed using macroscopy, microscopy, biochemical tests, and polymerase chain reaction (PCR) amplification of the 16S rRNA gene. Out of the 120 food samples analyzed, 7 (5.8%) yielded growth of L. monocytogenes alongside other Listeria spp., with a high rate of L. monocytogene isolation from frozen chicken (3.5%) and cabbage (2.7%). Raw meat and yoghurt samples also yielded 1 (0.8%) growth of L. monocytogenes each. Through the examination of various food samples, it was identified that L. monocytogenes were present. This can be seen as an opportunity to take proactive measures to ensure the safety of consumers, particularly the elderly, immunocompromised individuals, and pregnant women in the study area. Minimizing the potential risk of L. monocytogenes can promote a healthier community.
References
Akya, A., Najafi, F., Moradi, J., Mohebiand, Z. and Adabagher, S. (2013). Prevalence of food contamination with Listeria spp. in Kermanshah, Islamic Republic of Iran. Eastern Mediterranean Health Journal 19: 474-477. DOI: https://doi.org/10.26719/2013.19.5.474
Altekruse, S. F., Cohen, M. L. & Swerdlow, D. L. (1997). Emerging foodborne diseases. Emerging Infectious Disease 3, 285–293. DOI: https://doi.org/10.3201/eid0303.970304
Amajoud, N., Leclercq, A., Soriano, J. M., Bracq-Dieye, H., El Maadoudi, M., Senhaji, N. S., Abrini, J. (2018). Prevalence of Listeria spp. and characterization of Listeria monocytogenes isolated from food products in Tetouan, Morocco. Food Control, 84, 436–441.
Amajoud, N., Leclercq, A., Soriano, J. M., Bracq-Dieye, H., El Maadoudi, M., Senhaji, N. S., Abrini, J. (2018). Prevalence of Listeria spp. and characterization of Listeria monocytogenes isolated from food products in Tetouan, Morocco. Food Control, 84, 436–441. DOI: https://doi.org/10.1016/j.foodcont.2017.08.023
Arslan, S., and Baytur, S. (2018). Prevalence and Antimicrobial Resistance of Listeria species and subtyping and virulence factors of Listeria monocytogenes from retail meat. Journal of Food Safety 39, e12578. DOI: https://doi.org/10.1111/jfs.12578
Buchanan, R.L., Gorris, L.G.M., Hayman, M.M., Jackson, T.C. and Whiting, R.C (2017). A review of Listeria monocytogenes: An update on outbreaks, virulence, dose-response, ecology, and risk assessments. Food Control 75, 1-13. DOI: https://doi.org/10.1016/j.foodcont.2016.12.016
Desai, A.N., Anyohac, A., Madoff, L.C. and Lassmann, B. (2019). Changing epidemiology of Listeria monocytogenes outbreaks, sporadic cases, and recalls globally: A review of ProMED reports from 1996 to 2018. International Journal of Infectious Disease 84, 48-53. DOI: https://doi.org/10.1016/j.ijid.2019.04.021
Gallegos, J.M., Vanegas, M.C., Albarracin, Y., Mattar, S., Poutou, R.A. and Carrascal, A.K. (2008) Frequency of isolation of Listeria species in different retail foods in Colombia. Animal Production Research and Advance 4: 9. DOI: https://doi.org/10.4314/apra.v4i1.36420
Jabir Al-Maliki, G.M. (2010) Prevalence of Listeria monocytogenes in frozen fish in Basrah city markets. Basrah Journal of Veterinary Research. 10: 127-132. Maria, A., Eliana, G., Alzira Maria M. B., Elaine, C. P. (2010). Quantification of Listeria monocytogenes in minimally processed leafy vegetables using a combined method based on enrichment and 16S rRNA real-time PCR. Food Microbiology 27; 19–23 DOI: https://doi.org/10.33762/bvetr.2010.55056
Jay, J.M. (2005). Prevalence of Listeria spp. In meat and poultry products. Food Control 7: 209-214. DOI: https://doi.org/10.1016/S0956-7135(96)00043-6
Kayode, A. J, and Okoh, A.I (2022) Assessment of multidrug-resistant Listeriamonocytogenes in milk and milk product and One Health perspective. PLoS ONE 17(7): e0270993. https://doi.org/ 10.1371/journal.pone.0270993 DOI: https://doi.org/10.1371/journal.pone.0270993
Kyoui, D., Takahashi, H., Miya, S., Kuda, T., Kimura, B., 2014. Comparison of the major virulence-related genes of Listeria monocytogenes in Internalin A truncated strain36-25-1 and a clinical wild-type strain. BMC Microbiology. 14, 15. DOI: https://doi.org/10.1186/1471-2180-14-15
Luchansky, J. B., Chen, Y., Porto-fett, A. C. S., Pouillot, R., Shoyer, B. A., Rycke, R. J. E., Dennis, S. (2017). Survey for Listeria monocytogenes in and on ready-to-eat foods from retail establishments in the United States (2010 through 2013): Assessing potential changes of pathogen prevalence and levels in a decade. Journal of Food Protection, 80(6), 903–921. DOI: https://doi.org/10.4315/0362-028X.JFP-16-420
Mahendra, P., Selamawit, M., Nigus, Z., Gebrerufael, G., Savalia, C.V., Gobu, B. (2017). Listeria Monocytogenes as an emerging Global Food-borne Zoonotic Bacterial Pathogen. Beverage & food world. 44 (3) 11-14.
Mead, P. S., Slutsker, L., Dietz, V., McCaig, L. F., Bresee, J. S., Shapiro, C., Griffin, P. M. & Tauxe, R. V. (1999). Food-related illness and death in the United States. Emerging Infectious Disease 5, 607–625. DOI: https://doi.org/10.3201/eid0505.990502
Mohamed, R. I., Abdelmonem, M. A., & Amin, H. M. (2018). Virulence and antimicrobial susceptibility profile of Listeria monocytogenes isolated from frozen vegetables available in the Egyptian market. African Journal of Microbiology Research, 12(9), 218–224 DOI: https://doi.org/10.5897/AJMR2018.8794
Mona, M. H., Samia, A. A., Asmaa M. M., Hagar, A. A., Yasser, M. M. (2019). Molecular Identification of Listeria Species from Some Processed Meat Products Using Specific Iap Gene and 16S rRNA Gene. Alexandria Science Exchange Journal, 40 (2). DOI: https://doi.org/10.21608/asejaiqjsae.2019.36518
Mook, P., O’Brien, S.J. and Gillespie, I.A. (2011). Concurrent conditions and human listeriosis, England, 1999–2009E. merging Infectious Diseases,17(1), 38–43. DOI: https://doi.org/10.3201/eid1701.101174
Muhammad, S. Iqra, M. Muhammad, H. Zeeshan, A. B. Ishrat. Y. (2020). A Mini-Review on Commonly Used Biochemical Tests for Identification of Bacteria. International Journal of Research Publications 54, (1), ISSN number 2708-3578 (Online) DOI: https://doi.org/10.47119/IJRP100541620201224
Nayak, D. N., Savalia, C.V., Kalyani, I. H., Kumar, R., Kshirsagar, D. P. (2015). Isolation, identification, and characterization of Listeria spp. from various animal origin foods, Veterinary World 8(6): 695-701. DOI: https://doi.org/10.14202/vetworld.2015.695-701
Ntshanka, Z., Ekundayo, T.C., du Plessis, E.M., Korsten, L., and Okoh, A.I. (2022). Occurrence and Molecular Characterization of Multidrug-Resistant Vegetable-Borne Listeriamonocytogenes Isolates.Antibiotics, 11, 1353. DOI: https://doi.org/10.3390/antibiotics11101353
Owuama, C. I. (2015). Enumeration of microorganisms. Microbiology Laboratory Manual, Microtrend Digital Press Yola. pp 30-33 ISBN978-978-943-328-2.
Oyalemi, L. A., Aboaba, O. O. and Banjo, O. A. (2018). Prevalence and antibiotics susceptibility profile of Listeria monocytogenes isolated from processed and unprocessed meat products. Federal University Lafia Journal of Science and Technology 4 (1) 55-61.
Peter, A., Umeh, E., Azua, E., & Obande, G. (2016). Prevalence and antimicrobial susceptibility of Listeria monocytogenes isolated from beef, pork, and chicken sold in Makurdi Metropolis. British Microbiology Research Journal, 14(5), 1–7. DOI: https://doi.org/10.9734/BMRJ/2016/25663
Reza, R., and Mehrdad, H. (2018). Epidemiology of Listeria monocytogenes prevalence in foods, animals and human origin from Iran: a systematic review and meta-analysis. BMC Public Health 18:1057. DOI: https://doi.org/10.1186/s12889-018-5966-8
Singh, R., Kaur, S. and Bedi, J.S. (2019). Listeria contamination in Chevon and Mutton from Retail Meat Shops and Slaughter House Environment of Punjab, India. FEMS Microbiology Letter 366. DOI: https://doi.org/10.1093/femsle/fnz111
Soni, D.K., Singh, M., Singh, D.V., Dubey, S.K., 2014. Virulence and genotypic characterization of Listeria monocytogenes isolated from vegetable and soil samples. BMC Microbiology. 14, 241. DOI: https://doi.org/10.1186/s12866-014-0241-3
Tolba, M., Tlili, M.U., Eichenseher, C., Loessner, M.J., Zourob, M., 2012. A bacteriophage endolysin-based electrochemical impedance biosensor for the rapid detection of Listeria cells. Analyst 137, 5749–5756. DOI: https://doi.org/10.1039/c2an35988j
Usman, U. B., Kwaga, J. K. P., Kabir, J., & Olonitola, O. S. (2016). Isolation and antimicrobial susceptibility of Listeria monocytogenes from raw milk and milk products in Northern Kaduna State, Nigeria. Journal of Applied& Environmental Microbiology, 4, 46–54. DOI: https://doi.org/10.1155/2016/4313827
Uta, G. Denise, H. Philip, M. H. (2005). Methods for the isolation and identification of Listeria spp. and Listeria monocytogenes: a review FEMS Microbiology Reviews 29; 851–875 DOI: https://doi.org/10.1016/j.femsre.2004.12.002
Vitals, A. I., Aguadoes, V.D and Garcia-Jalon, J.E. (2004). Occurrence of Listeria monocytogenes in fresh and processed foods in Navara, Spain. Internal Journal of Food Microbiology 90: 349-356. DOI: https://doi.org/10.1016/S0168-1605(03)00314-3
Walker, R. L. (1999). Listeria. In: Veterinary Microbiology, Hirsh D.C. & Zee Y.C., eds. Blackwell Science, Malden, Massachusetts, USA, 225–228.
Wang, Y. J. I, Li, Q. S. and Liu, M. (2021). Prevalence and Genetic Diversity of Listeria monocytogenes Isolated from Retail Pork in Wuhan, China. Frontier in Microbiology 12:620482. doi: 10.3389/fmicb.2021.620482 DOI: https://doi.org/10.3389/fmicb.2021.620482
World Health Organization, WHO. (2018b). Listeriosis. Retrieved from https://www.who.int/news-room/fact-sheets/detail/listeriosis.
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