INVITRO MEMBRANE PROTECTION EFFECT OF AGERATUM CONYZOIDES METHANOLIC EXTRACT ON HUMAN SICKLED CELLS

Authors

  • M. D. Ibrahim
  • S. E. Atawodi
  • A. B. Sallau

DOI:

https://doi.org/10.33003/fjs-2023-0706-2175

Keywords:

Ageratum conyzoides, In vitro, Red Blood Cells, Membrane protection, Sickle Cell Anemia

Abstract

Hemolytic anemia caused by membrane damage is the major objective of treating sickle cell anemia in both mainstream and alternative medicine, which is a characteristic hallmark of the disease.One plant that is reportedly utilized in traditional medicine to treat sickle cell anemia is ageratum conyzoides.  Prelimnary phytochemical investigation were carried out  according to the standard protocols. In vitro spectrophotometric analysis was used to assess the methanolic extract of A. conyzoides' capacity to shield sickle red blood cells from lysis.  Preliminary phytochemical screening of crude extracts of leaf, stem and root of Ageratum conyzoides tested positive for the presence of phytochemicals such as alkaloids, flavonoids, tannins, cardiac glycosides, saponins, steroids and triterpenes. The leaf extract caused significant (P<0.05) membrane protection with the value of 24.13±0.47% at 2.5mg/ml concentration, while the stem extract produced a significant (P<0.05) protective effect with the value of  29.13±0.47% at 2.5mg/ml.The root also gave a significant (P<0.05)  membrane protective effect  of 40.73±1.04%  at 2.5mg/ml. These finding clearly support the traditional usage of A. conyzoides in the management of sickle cell anemia by demonstrating the functions that the plant's leaves, stem, and roots play in membrane protection.

References

Boampong, J. N., Karikari, A. A., Elvis, O. (2015). In vivo Antiplasmodial and in vitro Antioxidant Properties of Stem Bark Extracts of Haematostaphis barteri. Asian Journal of Tropical Biomedicine, 5 (6): 446 – 450

Ezekiel, J. S.; Adamu, h. m.; Chindo, I. Y.; Garba, i. h. (2015). Phytochemical Profile and Antioxidant Activities of Solvent-solvent Fractins of Haematostaphis barteri Hook f. (Anacardeceae) Stem Bark Extracts. International Journal of Pharmaconosy and Phytochemical Research, 8(1): 51-56. www.ijppr.com

Kubmarawa D., Ajoku G. A., Nwerem N. M. and Okorie D. A. (2007). Preliminary Phytochemical and Antimicrobial Screening of 50 Medicinal Plants from Nigeria. African Journal of Biotechnology, 6 (14): 1690 – 1696

Rabo, E. T. and Sanusi, S. S. (2001). An Inventory of Medicinal Plants of Nigeria Savannah, Jos: Leviathan Books. Pp 21- 24

Reach Devices, USA. Thin Layer Chromatograph (TLC) Stain. Retrieved in June 21, 2015, from www.reachdevices.com/TLC_stain.html

Schelenburg, I. and Kabrodt K. (2015). Identification of Phenolic Compounds in Rheum officinale Baill. by TLC-MS Coupling. Planar Chromatography in Practice. Retrieved in June 21, /2015 from http://issuu.com/camag/docs/cbs109_e

Wang, Y. Y., Jun-Main, T., Cheng-Chen, Z., Bo, L. and Jin-Ming, G. (2012). Sesquiterpene Glycoside and a Coumarin Derivative from Coriaria nepalensis and their Neurotropic Activity. Molecules, 21: 1344.

Wojciak-kosior, M. and Oniszczuk, A. (2008). Sample Preparation and TLC Analysis of Phenolic Acid. In Waksmundzka. H. M., Joseph S. and Kowalska T. Thin Layer Chromatography in Phytochemistry. Chromatographic Series Volume 99. Boca Raton, USA: Taylor and Francis Gp LLC: Pp 331-364

Wurochekke, A., Usman, B. H., Yusuf, H. B. and Mahmoud, S. J. (2014) In vitro Trypanocidal Activity of Aqueous Stem Bark Extract of Haematostaphis barteri Against Trypanosoms congelense and Trypanosoma brucei brucei. Scholars Academic Journal of Pharmacy, 3 (1): 38 - 42

Zhang, Q. W., Lin, L. G. and Ye, Wen – Cai (2018). Techniques for Extraction and Isolation of Natural Products: A Comprehensive Review. Chinese Medicine, 13: 20

Published

2024-01-30

How to Cite

Ibrahim, M. D., Atawodi, S. E., & Sallau, A. B. (2024). INVITRO MEMBRANE PROTECTION EFFECT OF AGERATUM CONYZOIDES METHANOLIC EXTRACT ON HUMAN SICKLED CELLS. FUDMA JOURNAL OF SCIENCES, 7(6), 104 - 109. https://doi.org/10.33003/fjs-2023-0706-2175