NUTRITIONAL CONTENT, PHYTOCHEMICALS AND IN-VITRO ANTIOXIDANT ACTIVITIES OF ETHANOL EXTRACT OF RED AND WHITE ONION
Abstract
Since antiquity onions (Allium cepa, L) have been cultivated across the globe as an important source of food and medicine. The study evaluated the nutritional content, phytochemistry and invitro antioxidant activities of ethanol extract of red and white onion bulbs. Red and white onion bulbs were purchased from a Local market in Maiduguri. Ethanol extracts were prepared using homogenized bulb. The proximate analysis, flavonoids, tannin, and phenol contents of the extracts as well as the antioxidant activities (total antioxidant activity, reducing power, and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities) were evaluated using standard procedures. The ash and carbohydrate contents of the two extracts were similar. However, the fat and protein contents were higher in white onion (2.44% & 1.05%) compared to the red onion (1.00% & 0.13%) while the moisture content was higher in red onion (16.10%) compared to the white onion (13.60%). The flavonoids, tannin, and phenol content as well as the total antioxidant activity was significantly higher (p<0.05) in the red onion relative to the white. White onion had a better reducing power activity compared to the red while the red had higher DPPH free radical scavenging activity compared to the white. Conclusively, our findings revealed that both red and white onion contains varying quantity of phenolic compounds with strong reducing power and DPPH free radical scavenging activity. However, red onion was shown to have higher antioxidant activity relative to the white.
References
Adeoti, O., Oyedele, O.A., & Yusuf, A. (2021). The water footprint of dry onion production in Nigeria. Water Resources and Industry, 25, 100147. https://doi.org/10.1016/j.wri.2021.100147 DOI: https://doi.org/10.1016/j.wri.2021.100147
Aksay, G., & Yavuzaslanoglu, E. (2023). Status of onion production in Turkiye and in the world, effects of abiotic and biotic stress factors. Anatolian Journal of batany, 7(1), 32-39. https://doi.org/10.30616/ajb.1240014 DOI: https://doi.org/10.30616/ajb.1240014
Alegbeleye, O., Odeyemi, O.A., Strateva, M., & Stratev, D. (2022). Microbial spoilage of vegetables, fruits and cereals. Applied Food Research 2, 100122. https://doi.org/10.1016/j.afres.2022.100122 DOI: https://doi.org/10.1016/j.afres.2022.100122
Aoyama, S., Yamamoto, Y. (2007). Antioxidant Activity and Flavonoid Content of Welsh Onion (Allium fistulosum) and the Effect of Thermal Treatment. Food Science and Technology Research, 13(1), 67-72. DOI: https://doi.org/10.3136/fstr.13.67
FAO (2020). Production, Crops, Onion, Area harvested, Yield, Production. http://www.fao.org/faostat/en/#data/QC [accessed December, 2023.
Floris, B., Galloni, P., Conte, V., & Sabuzi, F. (2021). Tailored Functionalization of Natural Phenols to Improve Biological Activity. Biomolecules, 11,1325. https://doi.org/10.3390/biom11091325 DOI: https://doi.org/10.3390/biom11091325
Hauner, H., Bechthold, A., Boeing, H., Brönstrup, A., Buyken, A., Leschik-Bonnet, E., Linseisen, J., Schulze, M., Strohm, D., & Wolfram, G. (2012). Evidence-Based Guideline of the German Nutrition Society: Carbohydrate Intake and Prevention of Nutrition-Related Diseases. Annals of Nutrition and Metabolism, 60(1):1-58. https://doi.org/10.1159/000335326 DOI: https://doi.org/10.1159/000335326
Ijeoma, W.C., Enemali, M.O., Asogwa, K.K., Onwukeme, C.A., & Nwodo, N.M. (2023). The Effect of Processing on the Antioxidant Activities of Purple Onions (Allium Cepa L.), Bulb. The Bio scientist Journal, 11(2), 194-207.
Jiang, X., & Kopp-Schneider A. (2014). Summarizing EC50 estimates from multiple dose-response experiments: A comparison of a meta-analysis strategy to a mixed-effects model approach. Biometrical Journal, 56(3), 493–512. https://doi.org/10.1002/bimj.201300123 DOI: https://doi.org/10.1002/bimj.201300123
Karu, E., Zhigila, D.A., Mohammed, A.B., Ismaila, M., Yuguda, U.A., & Farouk, M.U. (2017). Chemotaxonomic analyses and chemical content of some varieties of onion (Allium Cepa L.). Bima Journal of Science and Technology, 1(2), 183-193.
Kavalcová, P., Bystrická, J., Tóth, T., Trebichalský, P., Hrstková, M., Lenková, M., & Šiatkovský O. (2015). Content of total polyphenols and antioxidant activity in selected varieties of onion (Allium Cepa L.). Potravinarstvo Scientific Journal for Food Industry, 9(1), 494-500. https://doi.org/10.5219/524 DOI: https://doi.org/10.5219/524
Khan, A.U., Dagur, H.S., Khan, M., Malik, N., Alam, M., Mushtaque M. (2021). Therapeutic role of flavonoids and flavones in cancer prevention: Current trends and future perspectives. European Journal of Medicinal Chemistry Reports, 3, 100010. https://doi.org/10.1016/j.ejmcr.2021.100010 DOI: https://doi.org/10.1016/j.ejmcr.2021.100010
Kiura, I.N., Gichimu, B.M., & Rotich, F. (2021). Proximate and Nutritional Composition of Stored Bulb Onions as Affected by Harvest and Postharvest Treatments. International Journal of Agronomy, 5532349, 1-9. https://doi.org/10.1155/2021/5532349 DOI: https://doi.org/10.1155/2021/5532349
Kumar, M., Barbhai, M.D., Hasan, M., Punia, S., Dhumal, S., Radha, Rais, N., Chandran, D., Pandiselvam, R., Kothakota, A., Tomar, M., Satankar, V., Senapathy, M., Anitha, T., Dey, A., Sayed, A.A.S., Gadallah, F.M., Amarowicz, R., & Mekhemar, M. (2022). Onion (Allium cepa L.) peels: A review on bioactive compounds and biomedical activities. Biomedicine & Pharmacotherapy, 146, 112498. https://doi.org/10.1016/j.biopha.2021.112498 DOI: https://doi.org/10.1016/j.biopha.2021.112498
Kwon, Y-J., Lee, H.S., Park, J-Y., & Lee, J-W. (2020). Associating Intake Proportion of Carbohydrate, Fat, and Protein with All-Cause Mortality in Korean Adults. Nutrients, 12, 3208. https://doi/10.3390/nu12103208 DOI: https://doi.org/10.3390/nu12103208
Lee, Y.H., Candy Choo, C., & Waisundara, V.Y. (2015). Determination of the Total Antioxidant Capacity and Quantification of Phenolic Compounds of Different Solvent Extracts of Black Mustard Seeds (Brassica nigra), International Journal of Food Properties, 18:11, 2500-2507. https://doi.org/10.1080/10942912.2014.986331 DOI: https://doi.org/10.1080/10942912.2014.986331
Liga, S., Paul, C., & Péter, F. (2023). Flavonoids: Overview of Biosynthesis, Biological Activity, and Current Extraction Techniques. Plants, 12, 2732. https://doi.org/10.3390/plants12142732 DOI: https://doi.org/10.3390/plants12142732
Liguori, L., Califano, R., Albanese, D., Raimo, F., Crescitelli, A., & Di Matteo, M. (2017). Chemical Composition and Antioxidant Properties of Five White Onion (Allium cepa L.) Landraces. Journal of Food Quality, 6873651. https://doi.org/10.1155/2017/6873651 DOI: https://doi.org/10.1155/2017/6873651
Mahmood, N., Mauzzam, M.A., Ahmad, M., Hussain, S., & Javed, W. (2021). Phytochemistry of Allium cepa L. (Onion): An overview of its nutritional and pharmacological importance. Scientific inquiry and Review, 5(3), 41-59. https://doi.org/10.32350/sir/52 DOI: https://doi.org/10.32350/sir/53.04
Maleki, S.J., Crespo, J.F., Cabanillas, B. (2019). Anti-inflammatory effects of flavonoids. Food Chemistry, 299, 125124. https://doi.org/10.1016/j.foodchem.2019.125124 DOI: https://doi.org/10.1016/j.foodchem.2019.125124
Manye, S.J., Saleh, J.S., Ishaya, H.B., Chiroma, S.M., Attah, M.O.O., & Dibal, N.I. (2023). Phytochemical screening and in-vitro antioxidant activities of aqueous and methanol extracts of Aloe vera. Pharmacological Research-Modern Chinese Medicine 8, 100291. https://doi.org/10.1016/j.prmcm.2023.100291 DOI: https://doi.org/10.1016/j.prmcm.2023.100291
Moura de Melo, L.F., Aquino-Martins, V.G.Q., Pereira da Silva, A., Rocha, H.A.O., & Scortecci, K.C. (2023). Biological and pharmacological aspects of tannins and potential biotechnological applications. Food Chemistry, 414, 135645. https://doi.org/10.1016/j.foodchem.2023.135645 DOI: https://doi.org/10.1016/j.foodchem.2023.135645
Ochar, K., & Kim, S.-H. (2023). Conservation and Global Distribution of Onion (Allium cepa L.) Germplasm for Agricultural Sustainability. Plants 12, 3294. https://doi.org/10.3390/plants12183294 DOI: https://doi.org/10.3390/plants12183294
Roy, A., Khan, A., Ahmad, I., Alghamdi, S., Rajab, B.S., Babalghith, A.O., Alshahrani, M.Y., Islam, S., & Islam, M.R. (2022). Flavonoids a Bioactive Compound from Medicinal Plants and Its Therapeutic Applications. BioMed Research International, 5445291, 1-9. https://doi.org/10.1155/2022/5445291 DOI: https://doi.org/10.1155/2022/5445291
Ryan-Harshman, M., & Aldoori, W. (2006). New dietary reference intakes for macronutrients and fibre. Canadian Family Physician, 52, 177-179.
Sahlan, M., Hapsari, N.R.A., Pratami, K.D., Khayrani, A.C., Lischer, K., Alhazmi, A., Mohammedsaleh, Z.M., Shater, A.F., Saleh, F.M., Alsanie, W.F., Sayed, S., & Gaber, S. (2021). Potential hepatoprotective effects of flavonoids contained in propolis from South Sulawesi against chemotherapy agents. Saudi Journal of Biological Sciences, 28, 5461–5468. https://doi.org/10.1016/j.sjbs.2021.08.022 DOI: https://doi.org/10.1016/j.sjbs.2021.08.022
Shamsudin, N.F., Ahmed, Q.U., Mahmood, S., Shah, S.A.A., Sarian, M.N., Khattak, M.M.A.K., Khatib, A., Sabere, A.S.M., Yusoff, Y.M., & Latip, J. (2022). Flavonoids as Antidiabetic and Anti-Inflammatory Agents: A Review on Structural Activity Relationship-Based Studies and Meta-Analysis. International Journal of Molecular Sciences, 23, 12605. https://doi.org/10.3390/ijms232012605 DOI: https://doi.org/10.3390/ijms232012605
Shokri, S., Abdoli, N., Sadighara, P., Mahvi, A.H., Esrafili, A., Gholami, M., Behrooz Jannat, B., & Yousefi, M. (2022). Risk assessment of heavy metals consumption through onion on human health in Iran. Food Chemistry: X 14, 100283. https://doi.org/10.1016/j.fochx.2022.100283 DOI: https://doi.org/10.1016/j.fochx.2022.100283
Singh, B.N., Singh, B.R., Singh, R.L., Prakash, D., Singh, D.P., Sarma, B.K., Upadhyay, G., Singh, H.B. (2009). Polyphenolics from various extracts/fractions of red onion (Allium cepa) peel with potent antioxidant and antimutagenic activities. Food and Chemical Toxicology 47, 1161-1167. https://doi.org/10.1016/j.fct.2009.02.004 DOI: https://doi.org/10.1016/j.fct.2009.02.004
Smulikowska, S., Pastuszewska, B., ´Swiech, E., Ochtabinska, A., & Buraczewska, L. (2001). Tannin content affects negatively nutritive value of pea for monogastrics. Journal of Animal Feed Science, 10, 511-523. https://doi.org/10.22358/jafs/68004/2001 DOI: https://doi.org/10.22358/jafs/68004/2001
Tong, Z., He, W., Fan, X., & Guo, A. (2022). Biological Function of Plant Tannin and Its Application in Animal Health. Frontiers in Veterinary Science, 8,803657. https://doi.org/10.3389/fvets.2021.803657 DOI: https://doi.org/10.3389/fvets.2021.803657
Yeshiwas, Y., Alemayehu, M., & Adgo, E. (2023). The rise and fall of onion production; its multiple constraints on pre-harvest and post-harvest management issues along the supply chain in northwest Ethiopia. Heliyon 9, e15905. https://doi.org/10.1016/j.heliyon.2023.e15905 DOI: https://doi.org/10.1016/j.heliyon.2023.e15905
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