LACTIC ACID BACTERIA AND FERMENTED MAIZE SUPERNATANT (Omidun) HAVE ANTI-BIOFILM PROPERTIES AGAINST STAPHYLOCOCCI AND ENTEROAGGREGATIVE Escherichia coli STRAINS
DOI:
https://doi.org/10.33003/fjs-2023-0706-2134Keywords:
biofilm, lactic acid bacteria, pathogens, omidun, probioticsAbstract
Bacterial infections caused by biofilm forming organisms are of public health concern due to their propensity to contribute to persistent chronic diseases, chiefly because of their ability to resist antibiotics and host immune functions. Probiotics are considered useful therapeutic option in combating pathogenic biofilms. This study evaluates the anti-biofilm properties of potential probiotic Lactic Acid Bacteria (LAB) and fermented maize supernatant (Omidun) against selected biofilm-forming pathogens. Crystal violet biofilm assay was used to determine LAB and Omidun biofilm inhibition and dispersion in selected pathogens (Pseudomonas aeruginosa, Coagulase-negative staphylococci (CoNS), S. aureu and Enteroaggregative Escherichia coli) at different concentration (1%, 10%, 50%, 100%) of neutralized and non-neutralized cell free supernatant (CFS). Percentage biofilm inhibitions and dispersions were evaluated, and data were analysed with ANOVA. Omidun and LAB showed promising biofilm inhibitory and dispersive effect against the selected pathogens. L. plantarum showed the greatest biofilm inhibitory effect (P. aeruginosa: 7.85%, CoNS: 27.75%, S. aureus: 66.90%, EAEC: 39.73%) and dispersive effect (P. aeruginosa: 15.94%, CoNS: 23.27%, S. aureus: 24.90%, EAEC: 32.09%) against the selected pathogens while Omidun showed the least biofilm inhibitory and dispersive effect against the selected pathogens. There was no significance difference in the percentage of biofilm inhibition and dispersion produced under different concentrations, neutralized and non-neutralized state. Pseudomonas aeruginosa was the most resistant pathogen while Enteroaggregative Escherichia coli (EAEC) was the most susceptible. Inhibition and dispersion of biofilm can be mediated by LAB and Omidun, these effects appear to be independent of the produced organic acids
References
Abua, M. (2012). A comparative analysis of morphological and physicochemical characterization of soils of Southern Cross River State-Nigeria. Global Journal of Human Social Science Geography and Environmental Geo. Sciences, 12(13): 54–64
Adeyemi, A. A. and Adesoye, P.O. (2010). Site Quality Assessment and Yield Model for Tectona grandis (Linn. F.) stands in Ibadan metropolis. Nigerian. Journal of Forestry, 40(2): 67–77, 81, 13 –31. doi:10.1093/forestry/cpm041
Avery, T.E. and Burkhart, H.E. (2002). Forest Measurements, Journal 5thEdition McGraw-Hill Higher Education, New York, USA, 456:20
Bernstein, N., Gorelick, J., Zerahia, R. and Koch, S. (2019). Impact of N, P, K, and humic acid supplementation on the chemical profile of medical cannabis (Cannabis sativa L) Front Plant Sci, 10 (2019), p. 736
Bray R. H., and L. T. Kurtz. 1945. Determination of total, organic and available form of phosphorus in soil. Soil Soc. 59:39-45.
Clement, S.A (2023). Modeling Size-Density Relationship And Thinning Regime For The Management of Tectona Grandis Stands In Ado Teak Plantation, Ekiti State, Nigeria.FUDMA Journal of Sciences (FJS) Vol. 6 No. 2, April, 2022, pp 275 – 282
Dreyer, I., Gomez-Porras, J.L. and Riedelsberger, J. (2017). The potassium battery: A mobile energy source for transport processes in plant vascular tissues New Phytol, 216 (4) (2017), pp. 1049-1053
Flora of China Editorial Committee, (2016). Flora of China. St. Louis, Missouri and Cambridge, Massachusetts, USA: Missouri Botanical Garden and Harvard University Herbaria. http://www.efloras.org/flora_page.aspx?flora_id=2
González, G., Moya R., Monge, F., Córdoba, R. and Coto, J. C.(2004). Evaluating the Strength of Finger Jointed Lumber of Gmelina arborea in Costa Rica; Journal New Forest. 28: 319
Jose, S., Allen, S. C. and Nair, P. K. R. (2008). Tree-crop interactions: lessons from temperate alley-cropping systems. Ecological Basis of Agroforestry. CRC Press, Boca Raton Fl.15-36
KC, A., Bhandari, G., Wagle, S. P. and Banjade, Y. (2013). Status of soil fertility in a community forest of Nepal. International Journal of Environment, 1(1): 56-67
Lin, Z.H., Zhong, Q.S., Chen, C.S., Ruan, Q.C. and Chen, Z.H. (2016). Carbon dioxide assimilation and photosynthetic electron transport of tea leaves under nitrogen deficiency
Bot Stud, 57 (1) (2016), p. 37
López-Pintor A., Espigares, T., Rey-Benayas, J. M. and Gómez-Sal, A. (2000). Effect of simulated parent-created micro-environmental conditions on germination of Retama sphaerocarpa (L) Boiss seeds. Journal of Mediterranean Ecology 1:219–226
Lyam, A. A. (2000). Nigeria: A People United, A Future Assured. Survey of States, Volume (2), Gabumo Publishing, Calabar.
Paudel, S. and Sah, JP. (2003). Physiochemical Characteristics of Soil in Tropical Sal (S.robusta Gaertn.) Forest in Eastern Nepal. Himalayan.Journal of Sciences,1(2): 107-110
Poirier, Y. and Bucher, M. (2002). Phosphate transport and homeostasis in Arabidopsis Arabidopsis Book, 1 (2002), 0024
Reddy, E. N. V., Devakumar, A. S., CharanKumar, M. E. and Madhusudana, M. K. (2012). Assessment of Nutrient Turnover and Soil Fertility of Natural Forests of Central Western Ghats. International Journal of Science and Nature, 3(1): 5
Schmierer, M., Knopf, O. and Asch, F. (2021). Growth and photosynthesis responses of a super dwarf rice genotype to shade and nitrogen supply Rice Sci, 28 (2) (2021), pp. 178-190
Schoenholtz, S.H., Van-Miegroet, H. and Burger, J. A. (2000). A review of chemical and physical properties as indicators of forest soil quality: challenges and opportunities. Forest Ecology and Management 138, 335–356
Tiwari, K. R., Sitaula, B. K., Børresen, T. and Bajracharya, R. M. (2006). An assessment of soil quality in Pokhare Khola watershed of the Middle Mountains in Nepal. Journal of Food, Agriculture and Environment, 4(3&4): 276-283
Wright, S. J., Yavitt, J. B., Wurzburger, N., Turner, B. L., Tanner, E. V. J., Sayer, E. J., Santiago L. S., Kaspari, M., Hedin, L. O., Harms K. E., Garcia, M. N. and Corre, M. D. (2011). Potassium, phosphorus, or nitrogen limit root allocation, tree growth, or litter production in a lowland tropical forest. Ecology, 92(8): 1616-1625
Wang, Z. (2004). Cultivation and Utilization of Gmelina arborea in South Yunnan, China. New Forests, 28, 201.
Walkley, A. & Black I.A. (1934). An examination of the Degtjareff Method for Determining Soil Organic Matter, and a proposed Modification of the Chromic Acid Titration Method. Soil Science, 37(1):29-38
Wang, Y., Chen,Y.F. and Wu, W.H. (2021). Potassium and phosphorus transport and signaling in plants. J Integr Plant Biol, 63 (1) (2021), pp. 34-52
Ye, T.H., Li, Y.W., Zhang, J.L., Hou,W.F., Zhou,W.F., Lu, J.W., Xing, Y.Z. and Li, X.K. (2021b). Nitrogen, phosphorus, and potassium fertilization affects the flowering time of rice (Oryza sativa L.) Glob Ecol Conserv, 20 (2019), p. e00753
Zhu, Y., Fan, X.Y., Hou, X, C., Wu, J.Y. and Wang T. (2014). Effect of different levels of nitrogen deficiency on switchgrass seedling growth Crop J, 2 (2014), 223-234
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