PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL STUDIES OF THE LEAF EXTRACT OF COMBRETUM LAMPROCARPUM DIELS (COMBRETACEAE)
The aim of this study was to determine the antibacterial activity of Combretum lamprocarpum leaf extracts on some clinically isolated bacteria species. The pulverized plant material was extracted by microwave-assisted extraction using chloroform, ethyl acetate and methanol. The Agar well diffusion methods was used to determine the antibacterial activity of the plant against Salmonella typhi, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli. The phytochemical screening of the extracts revealed the presence of steroids, terpenes, alkaloids, carbohydrates, saponins, tannins, cardiac glycosides and flavonoids. The antibacterial screening of the extracts showed that all the fractions had significant activity against test organisms. The diameter of the zone of inhibition ranged between 14 and 29 mm, the minimum inhibitory concentration ranges between 3.13 and 50.00 mg/mL. The minimum bactericidal concentration was between 6.25 and 50.00 mg/mL. This study revealed that the leaves of Combretum lamprocarpum has a medicinal value in treating bacterial infections.
Ayoola, G. A., Coker, H. A., Adesegun, S. A., Adepoju-Bello, A. A., Obaweya, K., Ezennia, E. C. & Atangbayila, T. O. (2008). Phytochemical screening and antioxidant activities of some plants used for malaria therapy in South-western Nigeria. Tropical Journal of Pharmaceutical Research, 7(3), 1019-1024.
Cannell, R. J. (1998). How to approach the isolation of a natural products. Natural Products Isolation, Methods in Biotechnology. Humana Press, New Jersey, United States of America, Volume 4, pp: 1â€“51.
Eze, E. A., Oruche, N. E., Onuru, V. C. & Eze, C. N. (2013). Antibacterial screening of crude ethanolic leaf extracts of four medicinal plants. Journal of Asian Scientific Research,3(5), 431.
Gibreel, H. H. (2008), A Taxonomic Study on Trees and Shrubs of El Nour Natural Forest Reserve Blue Nile State- Sudan (unpublished masterâ€™s thesis). University of Khortoum, Sudan. pp. 76.
Ladan, Z., Amupitan, J. O., Oyewale, O. A., Ayo, R. G., Temple, E. & Ladan, E. O. (2014). Phytochemical screening of the leaf extracts of Hyptis spicigera plant. African Journal of Pure and Applied Chemistry, 8(5): 83-88.
Clinical and Laboratory Standard Institute (CLSI) (2014): Analysis and presentation of cumulative antimicrobial susceptibility test data. Retrieved from http://www.cid.oxfordjournals.org/content/44/6/867.fu
Offiah, N. V., Makama, S., Elisha, I. l. Makoshi, M. S., Gotep, J. G., Dawurung, C.J., Oladipo, O. O., Elisha, I. L., Samuel, A. L. & Shamaki, D. (2012). Survey of herbal remedies used by Fulani herdsmen in the management of animal diarrhoea in Plateau State, Nigeria. Journal of Medicinal Plants Research, 6(31): 4625â€“4632.
Petrovska, B. B. (2012). Historical review of medicinal plantsâ€™ usage. Pharmacognosy Reviews 6(11), 1.
Silva, G. L., Lee, I. S. & Kinghorn, A. D. (1998). Special problems with the extraction of plants. In: Cannel R.J.P. (eds) Natural Products Isolation. Methods in Biotechnology, vol 4. Humana press pp 343-363. DOI://doi.org/10.1007/978-1-59259-256-2_12
Sofowora, A. E. (1993). Medicinal Plants and Traditional Medicine in Africa. Ibadan, Nigeria: Spectrum Books Ltd. 55; 1-23.
Trease, G.E. & Evans M.C., (2002). Textbook of Pharmacognosy. 14th Edn. Balliere. Tindall London. pp. 81-90.
Trease, G.E. & Evans, W.O. (2009). Trease and Evans Pharmacognosy. 16th Edition. New York: Sauders Elsevier Limited. pp 104-262.
WHO (2005). National policy on traditional medicine and regulation of herbal medicines. Geneva: WHO Press
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