PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL STUDIES OF THE LEAF EXTRACT OF COMBRETUM LAMPROCARPUM DIELS (COMBRETACEAE)
Keywords:
Combretum lamprocarpum, Combretaceae, leaf extract, microwave-assisted extractionAbstract
The aim of this study was to determine the antibacterial activity of Combretum lamprocarpum leaf extracts on some clinically isolated bacteria species. The pulverized plant material was extracted by microwave-assisted extraction using chloroform, ethyl acetate and methanol. The Agar well diffusion methods was used to determine the antibacterial activity of the plant against Salmonella typhi, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli. The phytochemical screening of the extracts revealed the presence of steroids, terpenes, alkaloids, carbohydrates, saponins, tannins, cardiac glycosides and flavonoids. The antibacterial screening of the extracts showed that all the fractions had significant activity against test organisms. The diameter of the zone of inhibition ranged between 14 and 29 mm, the minimum inhibitory concentration ranges between 3.13 and 50.00 mg/mL. The minimum bactericidal concentration was between 6.25 and 50.00 mg/mL. This study revealed that the leaves of Combretum lamprocarpum has a medicinal value in treating bacterial infections.
References
Achieng, M. and Ruhode, E., (2013), “Challenges of Electronic Voting Technologies Within the South African Contextâ€, International Journal of Managing Information Technology (IJMIT), Vol. 5, No. 4, Nov 2013, Retrieved from www.airccj.org/ijmit
Agbu, O., (2015), “Unbridled Election Rigging and the Use of Technology: The Smart Card Reader as the ‘Joker’ in Nigeria’s 2015 Presidential Electionâ€, INEC Conference, 2015, Obtained from http://www.inecnigeria.org/wp-content/uploads/2015/07/Conference-Paper-by-Osita-Agbu.pdf
Akonjom, N. A. and Ogbulezie, J. C, (2014), “Application of contactless card reading technology for e- authentication of voters in Nigeriaâ€, IOSR Journal of Electronics and Communication Engineering (IOSR-JECE), Volume 9, Issue 2, Ver. VII (Mar - Apr. 2014), PP 31-37
Alebiousu, E. A., (2015), “Smart Card Reader and the 2015 General Elections in Nigeriaâ€, Journal of African Elections, Volume 15 Number 2 Oct 2016, Pp 69 – 89.
Ayinde, A. F. and Idowu, A. O., “Nigeria’s 2015 Elections: Permanent Voter’s Cards, Smart Card Readers and Security Challenges, Volume 15 Number 2 Oct 2016, Pp50 - 68.
Beaumont, R., (2012), An Introduction to Statistics Correlation, Obtained from http://www.robin- beaumont.co.uk/virtualclassroom/stats/course1.html, Accesed 22/6/2016
Bruck, S., Jefferson. D. and Rivest, R., (2010), “A Modular Voting Architecture (Frog Voting).â€, in ‘Towards Trustworthy Elections (Lecture Notes in Computer Science Series).’ Springer (2010), Retrieved from www.researchgate.net
Cass, A. G., Lerner, B. S., McCall, E. K., Osterweil, L. J., Sutton Jr., S. M., Wise, A., “Little-JIL/Juliette: (2000), A Process Definition Language and Interpreterâ€, Proceedings of the 22nd International Conference on Software Engineering. 4-11 June 2000. Limerick, Ireland. Pages 754 – 757.
Donald, S. Le Vie, Jr. (2000). “Understanding Data Flow Diagramsâ€, Proceedings of the 47th Annual Conference on Society for Technical Communication, Texas: Integrated Concepts, Inc
Erik Olin Wright and Joel Rogers, (2014), American Society: How It Really Works, W.W. Norton & Company, 2014 (Chapter 17)
European Commission/UNDP Joint Task on Electoral Assistance, (2012), “Summary Report of Thematic Workshop on Information Technology and Election Managementâ€, Mombassa ,5th – 9th March 2012, Retrieved from www.ec-undp-electoralassistance.org
Fairhurst, D. S., (2012), Using Excel for Business Analysis: A Guide to Financial Modeling, Plum Solutions
Galadanci, B. S., (2014). “ICT as a Tool for Election Management in Nigeriaâ€, Bayero Journal of Political Science, Maiden Edition, June 2014
Goldsmith, B. &Ruthrauf, H., (2013), Implementing and Overseeing Electronic Voting and Counting Technologies. IFES and NDI, , Retrieved from http://www.ifes.org/Content/Publications/Books/2013/Implementing-and-Overseeing-Electronic-Voting-and-Counting-Technologies.aspx
Hanewicz, C., (2012), Geographic Information Systems and the Political Process, Western Political Science Association Annual Meeting, March 2012, Obtained Fromhttps://wpsa.research.pdx.edu/meet/2012/hanewicz.pdf
IFES, (2015), IFES Nigeria Post-Election Survey, IFES, Obtained From www.ifes.org
INEC, (2011), Election Manual, Independent National Electoral Commission, Nigeria
INEC, (2015), Election Manual, Independent National Electoral Commission, Nigeria
Klein, R. I. &Merloe, P., (2001), Building Confidence In the Voter Registration Process: An NDI Monitoring Guide for Political Parties and Civic Groups, Obtained Fromwww.ndi.org.
Klimek, P., Yegorov, Y., Hanel, R and Thurner, S., (2012), Statistical Detection of Systematic Election Irregularities, Proceedings of the National Academy of Sciences, 109(41): 16469 - 16473
Kohno, T., Stubblefield, A., Rubin, A. D & Wallach, D. S., (2004), “Analysis of an Electronic Voting System, IEEE Symposium on Security and Privacyâ€, IEEE Computer Society Press, May 2004
Kuhne, W.,(2010), The Role of Elections in Emerging Democracies and Post Conflict Countries, Friedrich Ebert Stiftung
Leemann, L. &Bochsler, D., (2014), “A Systematic Approach to Study Electoral Fraudâ€, Electoral Studies, 35(2014):33-47, Elsevier, Obtained From http://dx.doi.org/10.1016/j.electstud.2014.03.0 05
NASS, (2010), “Electoral Act, Section 52 (2)â€, 2010, Obtained fromhttp://www.parliament.am/library/Electoral%20law/nigeria.pdf.
NDI, (2007), "Nigeria Final Report: Gubernatorial and State House Elections 14 April 2007 and Presidential and National Assembly Elections 21 April 2007," European Union Election Observation Mission
Pannell, D.J. (1997). Sensitivity analysis of normative economic models: Theoretical framework and practical strategies, Agricultural Economics 16: 139-152. Retrieved from dpannell.fnas.uwa.edu.au
SimidchievaB. I. et al., (2008), Specifying and Verifying Requirements for Election Processes, Proceedings of the 2008 international conference on Digital government research, Pages 63-72, 2008, Retrieved from http://www.umass.edu/eei/EEI Website Articles/Specifying and Verifying Requirements for Election Processes.pdf
Simidchieva, B. I.,(2010), “Modeling and Analyzing Faults to Improve Election Process Robustnessâ€, EVT/WOTE'10 Proceedings of the 2010 international conference on Electronic voting technology/workshop on trustworthy elections, 2010, Obtained from https://www.usenix.org/legacy/event/evtwote10/tech/full_papers/Simidchieva.pdf
Singhai, A. R., (2012), Technology Changes Elections, Science Reporter, June 2012, Obtained from www.niscair.res.in/sciencecommunication/
Thomas-Greenfield, (2015), "When Nigeria Decides, Nigeria Wins" – Press Release By U.S. Assistant Secretary of State for African Affairs Linda Thomas-Greenfield (April 20, 2015), Obtained from nigeria.usembassy.gov/press_releases.html
USAID, (2011), Biometrics in Elections, Georgia: De-Duplication or Voter Register and Verification of Voter Identity Using Biometics, Obtained from http://ifes.org/sites/default/files/biometrics_in_elections_2011_0.
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