SEPARATION OF ANTIOXIDANT COMPOUNDS FROM THE STEM BARK EXTRACTS OF HAEMATOSTAPHIS BARTERI HOOK F. (ANACARDIACEAE) USING CHROMATOGRAPHIC TECHNIQUES
In the search for plants with antioxidant properties, Haematostaphis barteri Hook f. (Anacardiaceae) was found to be a plant often used in Nigeria and other nations for the management of cancer, anemia, haemorrhoid and many other ailments. The ethyl acetate and acetone extracts of the stem bark in previous research by the same author were assayed for phenolic compounds and the phenolic compounds were found to possess antioxidant properties when TLC chromatograms were stained with iodine vapour and several spots were revealed. The ethyl acetate and acetone fractions were further chromatographed on TLC with several solvent systems and the mixtures from toluene, ethyl acetate and formic acid (TEF) in the ratio of 4/2.5/3.5 and 3/5/2 and n-butanol, acetic acid and water (BAW) in the ratio of 12/4/6 gave better resolutions. When chromatograms were obtained and developed with a solution of p-anisaldehyde in concentrated sulphuric acid, different spots were visualizedwith different colours signifying the presence of different bioactive metabolites. The Presence of antioxidant compounds was determined by bioutography TLC chromatograms were stained with a solution of 0.2% DPPH (2, 2-Diphenyl-1-picrylhydrazyl) solution in methanol and dried with heat gun and the appearance of yellow spots on purple background signifies the presence of antioxidants. A selected spot with an Rf value of 0.31 was monitored and isolated from column chromatography. The purified bioactive metabolite was crystallized, weighed and saved for further analysis.
Boampong, J. N., Karikari, A. A., Elvis, O. (2015). In vivo Antiplasmodial and in vitro Antioxidant Properties of Stem Bark Extracts of Haematostaphis barteri. Asian Journal of Tropical Biomedicine, 5 (6): 446 – 450
Ezekiel, J. S.; Adamu, h. m.; Chindo, I. Y.; Garba, i. h. (2015). Phytochemical Profile and Antioxidant Activities of Solvent-solvent Fractins of Haematostaphis barteri Hook f. (Anacardeceae) Stem Bark Extracts. International Journal of Pharmaconosy and Phytochemical Research, 8(1): 51-56. www.ijppr.com
Kubmarawa D., Ajoku G. A., Nwerem N. M. and Okorie D. A. (2007). Preliminary Phytochemical and Antimicrobial Screening of 50 Medicinal Plants from Nigeria. African Journal of Biotechnology, 6 (14): 1690 – 1696
Rabo, E. T. and Sanusi, S. S. (2001). An Inventory of Medicinal Plants of Nigeria Savannah, Jos: Leviathan Books. Pp 21- 24
Reach Devices, USA. Thin Layer Chromatograph (TLC) Stain. Retrieved in June 21, 2015, from www.reachdevices.com/TLC_stain.html
Schelenburg, I. and Kabrodt K. (2015). Identification of Phenolic Compounds in Rheum officinale Baill. by TLC-MS Coupling. Planar Chromatography in Practice. Retrieved in June 21, /2015 from http://issuu.com/camag/docs/cbs109_e
Wang, Y. Y., Jun-Main, T., Cheng-Chen, Z., Bo, L. and Jin-Ming, G. (2012). Sesquiterpene Glycoside and a Coumarin Derivative from Coriaria nepalensis and their Neurotropic Activity. Molecules, 21: 1344.
Wojciak-kosior, M. and Oniszczuk, A. (2008). Sample Preparation and TLC Analysis of Phenolic Acid. In Waksmundzka. H. M., Joseph S. and Kowalska T. Thin Layer Chromatography in Phytochemistry. Chromatographic Series Volume 99. Boca Raton, USA: Taylor and Francis Gp LLC: Pp 331-364
Wurochekke, A., Usman, B. H., Yusuf, H. B. and Mahmoud, S. J. (2014) In vitro Trypanocidal Activity of Aqueous Stem Bark Extract of Haematostaphis barteri Against Trypanosoms congelense and Trypanosoma brucei brucei. Scholars Academic Journal of Pharmacy, 3 (1): 38 - 42
Zhang, Q. W., Lin, L. G. and Ye, Wen – Cai (2018). Techniques for Extraction and Isolation of Natural Products: A Comprehensive Review. Chinese Medicine, 13: 20
Copyright (c) 2023 FUDMA JOURNAL OF SCIENCES
This work is licensed under a Creative Commons Attribution 4.0 International License.
FUDMA Journal of Sciences