DIVERSITY OF MICROBES IN SOIL AGGREGATE FRACTIONS UNDER DIFFERENT LAND-USE IN NORTHERN GUINEA SAVANNA, NIGERIA
DOI:
https://doi.org/10.33003/fjs-2020-0402-192Keywords:
Soil aggregate fractions, microbes, land-use, diversity and populationAbstract
Microorganisms play vital role in keeping soil healthy. Bacteria, Fungi and Actinobacteria are active in degrading soil organic matter which improves soil ecosystem functioning. There exists a dearth of information on the influence of land-use on diversity of microbes in different soil aggregate sizes in Northern Guinea Savanna of Nigeria. This study assessed the diversity of soil microbes under six different land-use practices namely forest, fallow, pasture legume, pasture cereal, date palm plantation and continuous cultivated. In each land-use soil samples were collected at two depths (0-5 cm and 5-20 cm). The soil samples were divided into two parts, one part was left as collected from the field sites (bulk soil) and the other part was separated into three aggregate fractions (>250 µm, >53 µm and <53 µm). Bacteria, Fungi and Actinobacteria were cultured, isolated and identified, and some soil chemical properties were determined. Colony forming units per gram of soil (CFU/g soil) was computed for the soil microbes. Results revealed that Bacteria is the predominant soil microbe followed by Actinobacteria and then Fungi in all the land-uses. Bacteria population ranged from 1.49x107to 8.65x107CFU/g soil, Actinobacteria population ranged from 9.32x105 to 5.85x106 CFU/g soil and Fungi population ranged between 6.75x104 and 4.21x105 CFU/g soil. Higher microbial population were observed in forest soil than soils of the other land-uses. Continuously cultivated land had the least microbial population. Silt + clay soil fraction had significantly higher bacteria while the bulk soil was significantly richer in fungal population.
References
Abdel-Moshen, S.I., Heba, I.A., and Manal, M.H. (2016). A safe potential juice clarifying pectinase for Trichoderma viride EF-8 utilizing Egyptian onion skins. Journal of Genetic Engineering and Biotechnology, 14: 153-159
Hatice, A.M. and Filiz, U.T. (2016). Extracellular pectinase production and purification from a newly isolated Bacillus subtilis strain. International Journal of Food Properties, 19(11): 2443-2450
Islam, S., Feroza, B., Alam, M.R. and Begum, S. (2013). Pectinase production by Aspergillus niger isolated from decomposed apple skin. Bangladesh Journal of Scientific and Industrial Research, 48(1): 25-32
Karanukaran, S., Saravanan, A., Dhanasekaran, A., Senbagam, D. and Benthil, K. (2014). Xylanase production by Aspergillus niger. International Journal of ChemTech Research, 6: 4207-4211. ISSN: 0974-4290
Mohammed, J.I. (2013). Screening of fungi isolated from environmental samples for xylanase and cellulose production. ISRN Microbiology, 2013: Article ID 283423. https://doi.org/10.1155/2013/283423
Olaoluwa, O., Abiodun, I., Isiah, E. and Abiodun, O. (2018). Isolation and screening of xylanolytic fungi from soil of botanical garden: xylanase production from Aspergillus flavus and Trichoderma viride. Journal of Microbiology Research, 8(1): 9-18
Oliyad, J. O. and Dawit, A. (2018). Screening and molecular identification of pectinase producing microbes from coffee pulp. Biomed Research International, 2018:2961767.
Patrick, C.Y., Antonio, H.Y., Susanna, K.P. and Kwok-Yung, Y. (2010). A novel method of slide preparation for preservation of native fungal structures for microscopic examination and long term storage. Journal of Clinical Microbiology, 48(9):3053-3061
Raju, E.V. and Divakar, G. (2013). Screening and isolation of pectinase producing bacteria from various regions in Bangalore. International Journal of Research in Pharmaceutical and Biological Sciences, 4: 151-154
Rakesh, K. S. and Kavita, S. (2014). Isolation, Screening and Identification of Fungi from soil. International Journal of Scientific Research. 3(7): ISSN: 2277-8129
Ramachandran, S. and Kurup, G. (2013). Screening and isolation of pectinase from fruit and vegetable wastes and the use of orange waste as a substrate for pectinase production. International Research Journal of Biological Sciences, 2(9): 34-39
Rohit, K.C., Sudipta, K.M., Purushotham, B. and Kumara, S.M. (2013). Isolation, production and characterization of extracellular pectinase from Aspergillus niger K3. International Journal of Pharmaceutical and Biological Sciences, 4(4): 667-675. ISSN 0975-6299
Torimiro, N. and Okonji, R.E. (2013). Comparative study of pectinolytic enzyme production by Bacillus subtilis. African Journal of Biotechnology, 12:6498-6503
Published
How to Cite
Issue
Section
FUDMA Journal of Sciences