THE EFFECTS OF FOLIAR APPLICATION OF SOME SELECTED PHYTOHORMONES ON THE PROXIMATE, PHYTOCHEMICAL, AND MINERAL COMPOSITIONS OF ROSELLE (HIBISCUS SABDARIFFA L.) LEAVES

  • Olajide M. Keshinro Lagos State University
  • Abdulazeez A. Ogbe Lagos State University, Ojo, Lagos
  • Khadijat Y. Oreshile
  • Kehinde T. Omolokun
Keywords: foliar spray, phytohormones, phytochemical, roselle, proximate

Abstract

Recent research has shown that agricultural crops can be improved through the use of phytohormones. In this study, we tested the effects of coconut water (CW), Gibberellic Acid (GA3), Indole Acetic Acid (IAA), 6-Benzylaminopurine (BAP), and Naphthalene Acetic Acid (NAA) through foliar spray, on the seedlings of Hibiscus sabdariffa L. (Roselle) to understand the best treatment to improve nutritional quality. Roselle seedlings were raised in 30cm × 19cm polythene bags and arranged using a completely randomized design in a screened house for 4 months. The phytohormones were applied as treatments on seedlings both singly and combined in the range of 10-20 (%) for CW and 50-200 (mg/L) for the other treatments. Proximate, vitamin C and mineral elements were determined using standard analytical procedures, while phytochemical screening was carried out on plant extracts. The best nutritional composition was achieved using 200mg/L GA3 and 20% CW with crude protein, fibre, vitamin C, and flavonoid contents of 4.17, 1.36, 46.7, and 3.72 mg/100g respectively. Treatments with 20% CW also recorded the highest calcium and magnesium contents of 193.20 and 89.20 mg/100 g respectively. Generally, the single hormone treatments outperformed the combined hormone treatments. The exceptional performance of CW proved that CW has the potential to improve the nutritional content of agricultural crops and can also serve as a cheap and accessible bio-stimulant for agricultural crops such as H. sabdariffa.

References

Anyasor, G. N., Aina, D. A., Olushola, M. and Aniyikaye, A. F. (2011). Phytochemical constituent, proximate analysis, antioxidant, antibacterial and wound healing properties of leaf extracts of Chromolaena odorata. Annals of Biological Research, 2:441-451.

A.O.A.C. (2005) Official Methods of Analysis of Official Analytical Chemistry. Pub. By the Association of Analytical Chemistry, Inc., Arlington, West Virginia, USA.

AOAC. (2015) Official Methods of Analysis of Official Analytical Chemistry. Pub. By the Association of Analytical Chemistry, Inc., Arlington, West Virginia, USA.

Borrás-Linares, I., Fernández-Arroyo, S., Arráez-Roman, D., Palmeros-Suárez, P. A., DelVal-Díaz, R., Andrade-Gonzáles, I., Fernandez-Gutierrez, A., Gomez-Leyva, J. F. and Segura-Carretero, A. (2015) Characterization of phenolic compounds, anthocyanidin, antioxidant and antimicrobial activity of 25 varieties of mexican roselle (Hibiscus sabdariffa). Industrial Crops and Products, 69:385-394. DOI: https://doi.org/10.1016/j.indcrop.2015.02.053

Boukerche, H., Malki, F., Saidji, N., Ghaliaoui, N., Bensalem, A. and Mokrane, H. (2023) Combination of ultrasound, microwave and conventional extraction techniques for roselle (Hibiscus Sabdariffa. L.) total anthocyanins and phenolics recovery: effect on antioxidant and structural properties. Biomass Conversion and Biorefinery. https://doi.org/10.1007/s13399-023-04029-8 DOI: https://doi.org/10.1007/s13399-023-04029-8

Buljeta, I., Pichler, A., Šimunovi´c, J., and Kopjar, M. (2022) Polysaccharides as carriers of polyphenols: comparison of freeze-drying and spray-drying as encapsulation techniques Molecules, 27:5069 DOI: https://doi.org/10.3390/molecules27165069

Ciuli, I. 1994. Methodology for analysis of vegetable drugs. Chemical Industries branch, division of Industrial operations. UNIDO, Romania: 24- 67 DOI: https://doi.org/10.1007/978-1-349-13185-3_14

Da-Costa-Rocha, I., Bonnlaender, B., Sievers, H., Pischel, I. and Heinrich, M. (2014) Hibiscus sabdariffa L.- A phytochemical and pharmacological review. Food Chemistry, 165: 424-443 DOI: https://doi.org/10.1016/j.foodchem.2014.05.002

Daudu, O. A. Y., Falusi, O. A., Dangana, M. C., Abubakar, A., Yahaya, S. A. and Abejide, D. R. (2015) Collection and evaluation of Roselle (Hibiscus sabdariffa L.) germplasm in Nigeria. African Journal of Food Science, 9(3):92-96 DOI: https://doi.org/10.5897/AJFS2014.1225

Eslaminejad, T. and Zakaria, M. (2011). Morphological characteristics and pathogenicity of fungi associated with Roselle (Hibiscus sabdariffa) diseases in Penang, Malaysia. Microbial pathogenesis, 51(5): 325-337. DOI: https://doi.org/10.1016/j.micpath.2011.07.007

Fadimu, O. Y., Ajiboye, A. A., Agboola, D. A., Kadiri, M. and Adedire, M. O. 2012. Effect of some combination of phytohormones on some growth parameters and vitamin C, carbohydarate, protein and chlorophyll contents of Spondias Mombin (Linn) seedlings. Ife Journal of Science, 14(2): 397-403.

FAO, WHO. Human vitamin and mineral requirements. Report of a Joint FAO/WHO Expert Consultation Bangkok, Thailand: Food and Nutrition Division FAO. 2001.

Hapsari, B. W., Manikharda, and Setyaningsih, W. (2021) Methodologies in the analysis of phenolic compounds in roselle (Hibiscus sabdariffa L.): composition, biological activity, and beneficial effects on human health. Horticulturae, 7: 35. DOI: https://doi.org/10.3390/horticulturae7020035

Hinojosa-Gómez, J., San Martin-Hernández, C., Heredia, J. B., León-Félix, J., Osuna-Enciso, T. and Muy-Rangel, M. D. (2018) Roselle (Hibiscus sabdariffa L.) cultivars calyx produced hydroponically: Physicochemical and nutritional quality. Chilean Journal of Agricultural Research, 78:4 doi :10.4067/S0718-58392018000400478 DOI: https://doi.org/10.4067/S0718-58392018000400478

Iqbal, M. A., Chaudary, M. N., Zaib, S., Imran, M., Ali, K. and Iqbal, A. 2011. Accumulation of Heavy Metals (Ni, Cu, Cd, Cr, Pb) in Agricultural Soils and Spring Seasonal Plants, Irrigated by Industrial Wastewater, Journal of Environmental Technology and Management, 2:3-12

ISTA (International Seed Testing Asspociation) (1976). International rules on seed testing. Seed Science and Technology, 4:3-94

Keshinro, M. O., Kadiri, M., Ojewumi, A. W. and Augustine, T. E. (2007). Vegetative growth analysis of Hibiscus sabdariffa L. (Roselle) treated with some plant growth substances. Direct Research Journal of Agriculture and Food Science, 5(12): 401-408.

Khan, M. E., Adebayo, K. O., Osigbemhe, I. G., Maliki, M., Bolaji, A. M., Paul, F. and Edeeje J. P. (2023) Comparative proximate composition, anti-nutritional analyses and ant-microbial screening of some Nigerian medicinal plants. FUDMA Journal of Sciences, 7(2):159-163. DOI: https://doi.org/10.33003/fjs-2023-0702-1722 DOI: https://doi.org/10.33003/fjs-2023-0702-1722

Odebiyi, O. O. and Sofowora, E. A. (1978). Phytochemical Screening of Nigerian Medicinal Plants. Lloydia, 41(3):234-246

Paraíso, C. M., dosSantos, S. S., Ogawa, C. Y. L., Sato, F., dos Santos, O. A. A., Madrona, G. S. (2020) Hibiscus sabdariffa L. extract: characterization (FTIR ATR), storage stability and food application. Emirates Journal of Food and Agriculture, 32(1):55-61 DOI: https://doi.org/10.9755/ejfa.2020.v32.i1.2059

Riaza, G. and Chopra R. (2018) A review on phytochemistry and therapeutic uses of Hibiscus sabdariffa L. Biomedicine & Pharmacotherapy, 102:575-586 DOI: https://doi.org/10.1016/j.biopha.2018.03.023

Robert, J. (2010) Food Analysis. West Lafayette, IN, USA: Purdue University.

Shakalenga, C. M., Mboni, H. M., Nkulu, S. N., Mavungu, G. N., Mwenya, C. K., Mwinensenge, J. M., Chiribagula, V. B., Amuri, S. B., Byanga, J. K. and Bualufu, V. N. B. (2021) Mineral elements analysis and total flavonoids content in the fresh leaves from two varieties of Hibiscus sabdariffa L. consumed as vegetable in Lubumbashi (DR Congo) World Journal of Advanced Research and Reviews, 9(1):147-155 DOI: https://doi.org/10.30574/wjarr.2021.9.1.0512

Sofowora, A. (2008) Medicinal plants and traditional medicine in Africa'. 3rd ed. Ibadan: Spectrum Books

Stella, T. R., Paraíso, C. M., Pizzo, J. S., Visentainer, J. V., Dos-Santos, S. S., and Madrona, G. S. (2023) Hibiscus (Hibiscus Sabdariffa L.) extracts freeze-dried and encapsulated by ionic gelation: an approach for yogurt application. Journal of Food Measurement and Characterization https://doi.org/10.1007/s11694-023-01818-7 DOI: https://doi.org/10.1007/s11694-023-01818-7

USDA, A. (2007). National Genetic Resources Program. In Taxon: Hibiscus sabdariffa L. Germplasm Resources Information Network-(GRIN) [Online Database]. Beltsville, Maryland: National Germplasm Resources Laboratory. http://www.ars-grin.gov/cgi-bin/npgs/html/taxon.pl?19078.

Usman, H. and Ismail, A. (2023) Phytochemical and antihelmintic studies of the leaves extract of Sida acuta Burm. against Taenia saginata. FUDMA Journal of Sciences, 7(2):223-226. DOI: https://doi.org/10.33003/fjs-2023-0702-1736 DOI: https://doi.org/10.33003/fjs-2023-0702-1736

Van-burden T. P. and Robinson W. C. (1981). Formation of complexes between protein and tannin acid. Journal of Agricultural and Food Chemistry, 1:77-82.

Witham, F. H., Blaydes, D. F. and Devlin. R. N. (1971) Experiments in plant physiology. D. Van Nostrand Comp. New York. pp. 55-56

Zhen, J., Villani, T. Y., Guo, Y., Qi, K., Chin, M. H., Pan, C.T., Ho, J. E., Simon, Q. and Wu, Q. (2015) Phytochemistry, antioxidant capacity, total phenolic content and anti-inflammatory activity of Hibiscus sabdariffa leaves, Food Chemistry 190: 673-680. DOI: https://doi.org/10.1016/j.foodchem.2015.06.006

Published
2023-06-30
How to Cite
Keshinro O. M., Ogbe A. A., Oreshile K. Y., & Omolokun K. T. (2023). THE EFFECTS OF FOLIAR APPLICATION OF SOME SELECTED PHYTOHORMONES ON THE PROXIMATE, PHYTOCHEMICAL, AND MINERAL COMPOSITIONS OF ROSELLE (HIBISCUS SABDARIFFA L.) LEAVES. FUDMA JOURNAL OF SCIENCES, 7(3), 249 - 256. https://doi.org/10.33003/fjs-2023-0703-1771