WATER SOLUBLE FRACTION OF METHANOLIC EXTRACTS OF CURCUMA LONGA (L.) ON THE ABSORPTION SPECTRUM OF DEOXYRIBONUCLEIC ACID
Keywords:
Curcuma longa; DNA; Methanolic extracts; Thin layer chromatography (TLC).Abstract
This study was carried out to examine the staining of fractions prepared from defatted methanolic extract of Curcuma longa (L.) as a natural product in interacting with nucleic acid of Calf Thymus DNA to ascertain the DNA binding property of the fractions using Thin Layer Chromatography. Dry powdered sample of Curcuma longa was subjected to cold maceration using 95% methanol. The cold liquid extract was concentrated with rotary evaporator and subsequently evaporated to dryness in water bath at 50ºC. The water soluble portion of the dark brown crude extract was partitioned subsequently in a separating funnel using solvents of varying polarity (chloroform, ethyl acetate, n-butanol.) Three dry fractions obtained from separating funnel system were further subjected to thin layer chromatography from where a total of 3 fractions were obtained. Each thin layer fraction (6.6×103µg/ml) were mixed with 13.5µg/ml of DNA and the absorption spectrum where compared with control. Of the three fractions obtained, three (100%) showed fluorescence when viewed under UV light. The effect of Thymus DNA observed for fraction 1A seems to be unique and may perhaps bind to Calf Thymus DNA. Fractions 2A and 2B showed loss of some peaks and bands which may be probably due to toxic effect on DNA or other mechanisms not presently clear. Thus we propose that fractions 1A, from preparative TLC of defatted methanolic extract of Curcuma longa, had effect on absorption spectrum of Calf Thymus DNA and could probably serve as basis for search of alternative DNA binding stain
References
Analysis of Profitability and Technical Efficiency of Fish Farming using Different Rearing Techniques in Nigeria. Asian Journalof Agricultural Extension, Economics & Sociology, 3(5): 405-418.
Ajao, A. M. (2012). Comparative Studies on Ecology and Morphometrics of Reared and Feral Honeybees in Geological ones of Kwara State, Nigeria. Phd Thesis. Federal University of Agriculture, Abeokuta.
Ajao , A. M., Oladimeji, Y. U., Babatunde, S. K. and Obembe, A. (2014). Differential Morphometric Patterns of Apis Mellifera and Adaptation to Climatic Variations in Kwara State, Nigeria. Global Journal of Bioscience and Biotechnology, 3(1): 34-40.
Ajao, A. M. and Oladimeji, Y. U. (2017). Farmers’ Knowledge, Attitude and Perception of Bee Pollination of Watermelon and Soybean in North-Central, Nigeria. Journal of Advances in Biology & Biotechnology, 12(1): 1-9.
Charlieveurel, C. (2017). Honey bee stressors. Retrieved from https://www.usda.gov/nass/PUBS. Retrieved 19/02/2018.
Gallai, N., Salles, J-M., Settele, J. and Vaissière, B. E. (2009). Economic Valuation of the Vulnerability of World Agriculture Confronted with Pollinator Decline. Ecological Economics, 68: 810-821.
Goodman, L. A. (1961). Snowball Sampling. Annals of Mathematical Statistics, 32: 148–170.
Haylegebriel, T. (2014). Honey Bee Diseases, Pest and their Economic Importance in Ethiopia. International Journal of Innovation & Scientific Research, 10(2): 527-535.
Kalanzi, K., Nansereko, S., Buyinza, J., Kiwuso, P., Turinayo, Y., Mwanja, C., Niyibizi, G., Ongerep, S., Sekatuba,J. and Denis Mujuni, D. (2015). Socio-economic Analysis of Beekeeping Enterprise in Communities Adjacent to Kalinzu Forest, Western Uganda. International Journal of Research on Land-use Sustainability, (2): 81-90.
Klein, A. M., Vaissiere, B. E., Cane, J. H., Steffan- De- wenter, I., Cunningham, S. A., Kremen C. and Tscha- rntke, T. (2007). Importance of Pollinators in Changing Landscapes for World Crops. Proceedings of Royal society of London, 274: 303-313.
Klein, S., Cabirol, A., Devaud, J., Barron, A. B. and Lihoreau, M. (2017). Why Bees are so Vulnerable to Environmental Stressors, Trends in Ecology and Evolution?. Tree 2201,11Pp.
Lawal, O. A and Banjo, A. D. (2010). Appraising the Knowledge and Perception of Pests Problems in Beekeeping Business at Different Ecological Zones in South Western Nigeria. World Journal of Zoology, 5(2): 137-142.
Michael. S., Hongmei, L., Ming H. H., Micheline, K. S., Olav, R. and David R. T. (2016). Migratory Management and Environmental Conditions affecting Lifespan and Oxidative Stress in Honey Bees. Scientific Reports.
Morse, R. A. and Calderone, N. W. (2000). The Value of Honeybees as Pollinator of U.S Crops. Bee Culture, 20: 1-15.
Naïla E., Jean-Marc, D. and Andrew B. B. (2012). General Stress Responses in the Honey Bee. Insects, (3): 1271-1298.
Oladimeji, Y. U., Abdulsalam, Z., Damisa, M. A. and Omokore, D. F. (2014). Estimating the Determinants of Poverty among Artisanal Fishing Households in Edu and Moro Local Government Areas of Kwara State, Nigeria. Agriculture & Biology Journal of North America, 4(4): 422.429.
Oladimeji, Y. U., Ajao, A. M., Abdulrahman, S., Suleiman, R. and Bolaji, A. M. (2016). Estimation of Efficiency Differentials in Honey Bee Enterprises: Implications for Higher Productivity in Kebbi and Kwara States of Nigeria. Gashua Journal of Irrigation and Desertification Studies, 2(2): 38-49.
Oladimeji1, Y. U., Ajao, A. M. and Abdulsalam, Z. (2017a). Arable Crop Farming and Adoption of Bee Pollination Services among Farming Households in Kwara State, Nigeria. Asian Journal of Agricultural Extension, Economics & Sociology, 15(2): 1-10
Oladimeji, Y.U., Ajao, A. M.,Abdullahi, A. N. , Abdulsalam, Z. and Damisa, M. A. (2017b). Adoption of Improved Technologies and Management Practices among Bee Farmers in North Central and North Western Nigeria towards Sustainable Development Goals. Ethiopia Journal of Applied Science and Technology, 8 (1): 1-13
Salganik, M. J. and Heckathorn, D. D. (2014). Sampling and Estimation in Hidden Populations Using Respondent-Driven Sampling. Sociological Methodology, 34 (1): 193–239.
Solomon, B. (2009). Indigenous Knowledge and its Relevance for Sustainable Beekeeping Development: A Case Study in the Highlands of Southeast Ethiopia. Livestock Research for Rural Development, 22(11): 11-18.
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