PHYTO CHEMICAL COMPOSITION AND In vitro ANTIPLASMODIAL POTENTIAL OF STEM BARK AND LEAF EXTRACTS OF Ficus gnaphalocarpa AND Ipomea fistulos USED IN YAURI AND ARGUNGU EMIRATES, KEBBI STATE, NIGERIA

Authors

Keywords:

Antiplasmodial activity, Plasmodium berghei berghei, Ficus gnaphalocarpa, Ipomea fistulos, malaria

Abstract

Malaria is the world’s most parasitic disease endemic in about 100 developing countries. The search for new antimalarial drugs from natural products is necessitated by resistance of the parasites to known antimalarial agents. This study  evaluated  phytocmical composition and the in vitro antiplasmodial potentials of Ficus gnaphalocarpa stem bark and Ipomea fistulos leaf methanolic extracts  obtained from two  areas (one each from Argungu and Yauri emirates of Kebbi State, Nigeria ) . The air dried samples of Ficus gnaphalocarpa (miq) C.C. Beng (stem bark) and Ipomea fistulos L. (leaves) were extracted with methanol for seventy two hours. The crude extracts were tested against chloroquine resistant strain of Plasmodium berghei berghei (NK-65) in vitro in 96 well microplates under anaerobic condition. The data obtained were analyzed using one way ANOVA and Duncan multiple comparison test with SPSS version 20.0. There was significant (P<0.05) reduction in the number of parasitized cells relative to control. F. gnaphalocarpa exhibited higher activity of 92.81% and IC50 (11.85 mg) while I. fistulos recorded an activity of 66.70% and IC50 (36mg). The plant extracts used in this study presented antiplasmodial activity. Further studies on in vivo assay, toxicity, histopathology, isolation and purification is recommended for possible development of their bio-constituents into antiplasmodial agents.

Dimensions

Adia, M. M., Emmani, S.N., Byamukama, R., Faye,I. and Borg-Kalson, A. (2016). Antiplasmodial activity and phytochemical analysis of extracts from selected Ugandan medicinal plants. Journal of Ethnopharmacology, 1-17. Available: http:11dxrdoi.org/10.1016/5j-jep.2016.03.047.

Anigboro, A.A.,Avwioroko, O.J. and Cholu, C.O. (2020). Phytochemical Constituents, Antimalaria Efficacy and Protective Effect of Eucalyptus camaldulensis Aqueous Leaf Extract in Plasmodium berghei infected Mice. Prev. Nutr.Food Sci.25 (1): 58-64

Alsiddig, O. and Sufyan, A. (2015). Phytochemical Screening of Ficus syncomorus. L Bark Extract and Cleome gynandral Aerial Parts. Journal of Phamacognosy and Phytochemicals, 4(54): 24-27.

Babandi, A., Muhammad, M.A., Anosike, C.A and Ezeanyika, L.U.S (2019).InvitroAntiplasmodial potential of chloroform Fruit Extract of Ficussycomorus L. against Blood-stagePlasmodium falciparum. Nigeria Research Journal of Chemical Sciences, 6:33 – 48.

Bello, O.N.., Zack, A.M. and Adikwu, J.G.G. (2013). Comprartive Studies of Phytochemical Screening of Ficus syncomorus Linn stem bark Extract and Pilliostigma thronningi Roots Extract. Pelagia research library, 3(6): 69-73.

Centre for Disease Control and Prevention. ‘Impact of Malaria: 2016. Available: WWW.cdc.gov/malaria/malariawor/idinde/impact.html.

Chinedu, E., Arome., D and Ameh, S.F. (2014). African Herbal Plants used as Anti-Malarial Agents – A Review. Pharmatutor, 2 (3): 47-53

Chand, N. and Rohatgi, P.K.(2005). Impact toughness of Ipomea carnea particulate-polyester composite. Journal of Material Science Letters, 6(6): 695-697. Retrieved from doi:10.1007/bfo1770929

Deguchi, J., Hirahara T., Hirasawa, Y., Ekasari, W., Widyawurayanti, A., Shirota, O. and Morita, H. (2012). New tricyclic alkaloids, cassiarins, G.H.J and K. from leaves of Cassia seanmea. Chemical and Pharmaceutical bulletin, 60(2); 219-222.

Evans, W. C. (1989). Trease and Evans Pharmacognosy. Thirteenth edition. ELBS Bailliere, Tindal, 532pp.

Gessler, M. C., Nkunya, M.H.N., Nwasumbi, L.B., Heinrich, M. and Tonner, M.(1994). Screening Tanzanian Medical Plants for antimalial Activity. Acta Tropical, 56: 65-67.

Harborne, J.B. (1984). Phytochemical Methods. A Guide to Modern Techniques in Plant Analysis. Chapman and Hall, London, p.279.

Hosseinzadeh, S., Jafarikukhdan, A., Hosseini, A., Armand, R. (2015). The application of medicinal plants in traditional and modern medicine: A review of Thymus vulgaris. International Journal of Chinical medicine: 635-642.

Kirmizibekmez, H., Calis, I., Peruzzo, R.I Donmez, A.A., Linden, A., Ruedi, P. and Tasdemir, D.(2004).Inhibiting activities of the secondary metabolites of Phlomis brunneogaleata against parasitic protozoal and plasmodialenoyl – ACP reductase, a crucial enzyme in fatty acid biosynthesis.PlantaMedica, 70 (8): 711-717.

Mbosso, T.J.E., Nguedia, A.J.C., Meyer, F., Donfack, V.E., Lenta, N.B., Ngouela, S., Tsamo, E., Adiogo, D., Azebaze, A.G.B. and wintjens, R. (2016b). Invitro antimicrobial and antiproliferative activities of plant extracts from Spathodea campanulata, Ficus bubu and Caricapapaya. Pharmaceutical Biology, 54:1086-1095.

Monbrison, F., Maitrejean, M., Latour, C., Bugnazet, F., Peyron, F., Berron, D. and Picot, S. (2006). In vitro antimalarial activity of flavonoid derivatives dehydrosilbin and 8-(1,1)- DMA Kaempferide. Acta Tropical, 97:102-107.

Muhammed, D., Dada, E.O., Muazu, M., Jumbo, E.I. and Uzokwe, V.I.(2018). Antiplasmodial Activity of Ethanolic leaf Extract of Eucalyptus citriodora in swiss Albino mice infected with P. berghei NK 65. South Asian Journal or Research in Microbiology, 2 (2): 1-10.

Mustofa, M.H., Sholikhah, E.N. and Wuhyuono, S. (2007). In vitro and in vivo antplasmodial activity and cytotoxicity of extracts of Phyllantus niruri L.traditionally to treat malaria in Indonesia. Southeast Asian Journal of Tropical Medicine and Public Health, 38(4): 609-615.

Ngemenya, M.N., Akam , T.M., Yong, J.N., Tane, P., Fansofree, S.N.Y., Berzins, K. and Titanji, V.P.K. (2006). Antiplasmodial activities of some products from Turrenthus africanus (meliaceae). African Journal of Health Sciences, 13: 33-39.

Ogundolie, O.O Dada,E.O., Osha, I.B. and Oloruntol, D.A. (2017). Effect of raw ethanolic seed extract of Tetracarpidium conophorumon mice infected with P. berghei. Journal of Applied Life Sciences International, 12: 1103-1134.

Olasehinde, G.I., Ayand O.I., Egwari I.O., Ajayi A.A and Awofeso T. (2016). In vivo Antiplasmodial Activity of Crude Ethanolic and N- hexane Extracts of Moringa oleifera Leaves. International Journal of Agriculture and Biology, 18:906-910.

Oyi, A.R., Onaolapo, J.A., Haruna, A. K. and Morah, C.O. (2007). Antimicrobial Screening and stability studies of crude extract of Jatropha Curcas Linn Latex (Euphorbiaceae). Nigerian Journal of Pharmaceutical Science, 6:14-20.

Phan, V.K., chau, V.M., Nguyen; Bui, H.T., Tran, H.Q., Hoang, L.T.A., Nguyen, X.C., Truong, N.A., Seung, H.K., Jin, K.K., Hae-Dong and young, A.K. (2012). Chemical Constituents of the Ficus elastic leaves and their antioxidant activities. Bulleting of Korean Chemical society, 33:3461-34664.

Rathnapala, U.L., Goodman, C.D. and Mcfadden, G. l.(2017). A novel genetic technique in Plasmodium berghei allows liver stage analysis of genes required for mosquito stage development and demonstrates that de novo heme synthesis is essential for liver stage development in malaria parasite. Plos pathog 13:1006396.

Salles, H., Vasconcelos, I.M., Santos, L.F.L and David de Olivera, H. (2011). Towards a better understanding of Ipomea asarifolia toxicity: Evidence of the Involvement of a leaf lecithin. Toxicon, 58 (6 – 7): 502-508.

Schanzt-Dunn, J. and Nour (2009). Malaria and pregnancy: A Global Perspective. Rev. Obstet Gynecol. 2009, 2 (3): 86-192.

Shija, K.M., Nondo, R.S.O., Mloka, D., Sangeda, R.Z. and Bwire, G.M. (2020). Effects of lemon decoction on malaria parasite clearance and selected haematological parameters in Plasmodium berghei ANKA infected mice. BMC complimentary medicine and Therapies, 20(24): 1-12.

Sofowora, A. (1993). In: Medicinal Plants and Traditional Medicine in Africa (2nd edition). John Wiley and sons Ltd, New York, p.85-198.

Tasdemir, D., Guner, N.D., Perozzo, R., Brun, R., Donmez, A.A., Calis, I. and Ruedi, P. (2005). Antiprotozoal and Plasmodial Fab I enzyme inhibiting metabolites of Scrophularia lepidota roots.Phytochemistry, 66(3): 355-362.

Trease, G.E. and Evans, W. C. (1989). Pharmacognosy, 11th edition, Bailliere, Tindal, London, p.60-75.

WHO (2017). Seasonal Malaria Chemoprevention (SMC).

WHO (2018). World Malaria: Fact Sheet 2018.

Zar, J.H. (1984). Biostatiscal Analysis, 2nd edition, Prentice-Hall International, Englewood Cliffs, N. J.

Published

23-08-2022

How to Cite

PHYTO CHEMICAL COMPOSITION AND In vitro ANTIPLASMODIAL POTENTIAL OF STEM BARK AND LEAF EXTRACTS OF Ficus gnaphalocarpa AND Ipomea fistulos USED IN YAURI AND ARGUNGU EMIRATES, KEBBI STATE, NIGERIA. (2022). FUDMA JOURNAL OF SCIENCES, 6(4), 144-149. https://doi.org/10.33003/fjs-2022-0604-1050

Issue

Vol. 6 No. 4 (2022): FUDMA Journal of Sciences - Vol. 6 No. 4

Section

Research Articles
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FUDMA Journal of Sciences

How to Cite

PHYTO CHEMICAL COMPOSITION AND In vitro ANTIPLASMODIAL POTENTIAL OF STEM BARK AND LEAF EXTRACTS OF Ficus gnaphalocarpa AND Ipomea fistulos USED IN YAURI AND ARGUNGU EMIRATES, KEBBI STATE, NIGERIA. (2022). FUDMA JOURNAL OF SCIENCES, 6(4), 144-149. https://doi.org/10.33003/fjs-2022-0604-1050