@article{Ehwarieme_Adomi_Ewhre_Akpamie_2022, title={ALCOHOL DISRUPTED AND INCREASED ANTIBIOTICS RESISTANT PROFILES OF SELECTED BACTERIA RESIDENT IN GASTRO-INTESTINAL TRACT OF WISTER RAT}, volume={6}, url={https://fjs.fudutsinma.edu.ng/index.php/fjs/article/view/981}, DOI={10.33003/fjs-2022-0603-981}, abstractNote={<p>The study is aimed at investigating the effect of alcohol intake on gut microbiota and antibiotic susceptibility of some of the microbial genera isolated. Twenty-four wistar rats were administered orally with branded dry gin (40% alcohol) and one was picked randomly every 48hrs and sacrificed by cervical decapitation. The intestine was collected aseptically after laparotomy and placed in a sterile petri dish. The intestinal bacteria were enumerated, isolated and identified using standard microbiological methods. The antibiotic susceptibility was done using the disc agar diffusion method. The Total Heterotrophic (x 10<sup>4</sup> CFU/g) and Total Coliform Counts ranged from 2.0 – 65 and 1 – 28 respectively. <u>Bacteria</u> genera isolated <u>are;</u> <em>Escherichia, Proteus, Citrobacter, Lactobacillus, Enterococcus, Enterobacter, Salmonella, Klebsiella</em> and <em>Bacillus.</em> <em>E. coli</em> was the most <u>(100%)</u> prevalent. <em>Enterococcus, Lactobacillus</em> and <em>Enterobacter</em> were also highly prevalent. The occurrence of the genera was in the order <em>Lactobacillus &gt; Citrobacter &gt; Klebsiella</em> and <em>Salmonella &gt; Proteus.</em> All isolates were present in all the intestines few days after administration of the gin, but there was a decline in the occurrence till the 21<sup>st</sup> day, after which there was an increase. The susceptibility of <em>Escherichia</em> to conventional antibiotics was between 0–90% and there was no particular trend with the length of exposure but there was substantial decrease in the susceptibility to the antibiotics. The susceptibility of <em>Enterococcus</em> to the antibiotics decreased with the length of exposure to the alcohol. Intake of alcohol has a pronounced effect on the gut microbiome and the antibiotic susceptibility of isolates.</p&gt;}, number={3}, journal={FUDMA JOURNAL OF SCIENCES}, author={EhwariemeD. A. and AdomiP. O. and EwhreO. L. and AkpamieO. O.}, year={2022}, month={Jun.}, pages={51 - 55} }